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Intercellular communication between hepatic stellate cells and myofibroblasts mediated by osteopontin and FGF18

ABSTRACT: Purpose: To further explore FGF18's role in liver fibrosis beyond its known effects on HSC proliferation and TGFβ signaling, we performed RNA sequencing on primary HSCs treated with FGF18 or TGF-b. Methods: Fresh isolated HSCs were starved with DMEM containing 0.2% FBS for 24 hours and then left untreated or stimulated with FGF18 (100 ng/mL), TGFb1 (1 ng/mL). Total RNA was extracted from HSCs using RNeasy Micro Kit (74004, QIAGEN). RNA libraries were prepared using the SMART-Seq HT PLUS Kit (R400748, Takara Bio) according to the manufacturer's protocol.Paired-end sequencing (2 × 150 bp) was performed on a NovaSeq 6000 system (Illumina) to generate approximately 40M reads (20M pairs) per sample, with a target of 6G bases per sample. Results: RNA-seq analysis revealed that FGF18 induced Ccnd1 while suppressing profibrotic genes such as Col1a2, Col3a1, and Acta2, whereas TGFβ showed opposite effects. Among 62 genes upregulated by both factors, we focused on Spp1, encoding osteopontin, given its known role in tissue fibrosis.

ORGANISM(S): Mus musculus

PROVIDER: GSE285364 | GEO | 2025/05/24

REPOSITORIES: GEO

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