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Single-Cell Transcriptomic Profiling of Xenotransplanted Retinal Organoids Derived from the WA09 CRX-tdTomato Human Embryonic Stem Cell Line

ABSTRACT: Single-cell gene expression profiles were generated for human retinal organoids derived from the WA09 CRX-tdTomato human embryonic stem cell line. Fragmented organoids were transplanted into the subretinal space of normal canine retinas and analyzed three days post-transplantation. In parallel, fragmented organoids from the same batch, maintained in culture for the same duration, were used as controls. Comparative single-cell transcriptomic analysis provided insights into the early molecular changes associated with the transplanted environment versus in vitro culture conditions, shedding light on the cellular responses and adaptation of retinal organoids post-transplantation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE285393 | GEO | 2025/06/01

REPOSITORIES: GEO

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