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PKM2/STAT1 pathway governs naïve B cell fate in germinal center formation within Peyer’s patches and gut mucosal immunity

ABSTRACT: This study investigates the effects of high-protein diets on intestinal B cell development and their role in Crohn’s disease (CD) pathogenesis. Our results demonstrate that a high-protein diet (HPD) combined with low-dose dextran sulfate sodium (DSS) effectively models CD in juvenile mice, leading to disrupted B cell development in Peyer’s patches (PPs). This is characterized by a reduction in germinal center (GC) B cells and an increase in plasma cells in PPs, accompanied by downregulation of the PKM2/STAT1 signaling pathway in B cells, as validated through single-cell RNA sequencing (scRNA-seq), BCR sequencing (scBCR-seq) and flow cytometry.

ORGANISM(S): Mus musculus

PROVIDER: GSE285427 | GEO | 2025/12/16

REPOSITORIES: GEO

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