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Nautilus null vs. wild type miRNA

ABSTRACT: To determine if the Drosophila MyoD homolog, nautilus, was activating any miRNA loci, similar to vertebrate MyoD, we compared the miRNA expression profiles between wild-type (w1118) and nautilus null embryos during the window of maximum nautilus expression (6-8hr AEL), using LNA arrays specifically designed to quantify miRNA levels in Drosophila (Exiqon). Expression levels for mir-309, mir-3, mir-286, mir-4, mir-5, and mir-6 from the 8-miR cluster, were significantly decreased in nautilus null embryos. It suggests that the intergenic 8-miR cluster, encoding eight miRNAs, is regulated by nautilus.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE28550 | GEO | 2015/04/01

SECONDARY ACCESSION(S): PRJNA143035

REPOSITORIES: GEO

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nautilus null vs. wild type miRNA

Project description:To determine if the Drosophila MyoD homolog, nautilus, was activating any miRNA loci, similar to vertebrate MyoD, we compared the miRNA expression profiles between wild-type (w1118) and nautilus null embryos during the window of maximum nautilus expression (6-8hr AEL), using LNA arrays specifically designed to quantify miRNA levels in Drosophila (Exiqon). Expression levels for mir-309, mir-3, mir-286, mir-4, mir-5, and mir-6 from the 8-miR cluster, were significantly decreased in nautilus null embryos. It suggests that the intergenic 8-miR cluster, encoding eight miRNAs, is regulated by nautilus. Two-condition experiment, wild type (w1118) vs. mutant (nautilus null). Biological replicates: 3 wild type, 3 mutants, independently isolated.

2015-04-01 | E-GEOD-28550 | biostudies-arrayexpress

8-miR cluster deletion affects mef2 levels in Drosophila melanogaster

Project description:Loss of nautilus (MyoD) gene function results in a variable phenotype affecting muscle formation in embryos and larvae, larval movement, pupal eclosion, egg deposition, adult mobility and survival. 8-miR cluster deletion disrupts muscle formation in the embryo while affecting protein production from the nautilus, dMef2 , regulators of the muscle transcriptional network. We propose the complex phenotype in the nautilus null is due to the disruption of the regulatory interactions provided by the 8-miR cluster. The results demonstrate that nautilus is an integral regulator of the miRNA circuitry buffering the transcriptional network directing muscle development.

2015-04-01 | GSE28604 | GEO

8-miR cluster deletion affects mef2 levels in Drosophila melanogaster

2015-04-01 | E-GEOD-28604 | biostudies-arrayexpress

Ectopic expression of mir-3 affects mef2 and tinman levels

Project description:Loss of nautilus (MyoD) gene function results in a variable phenotype affecting muscle formation in embryos and larvae, larval movement, pupal eclosion, egg deposition, adult mobility and survival. mir-3 over expression disrupts muscle formation in the embryo while affecting protein production from the dMef2 and tinman genes, global regulators of the muscle transcriptional network. We propose the complex phenotype in the nautilus null is due to the disruption of the regulatory interactions provided by the 8-miR cluster. The results demonstrate that nautilus is an integral regulator of the miRNA circuitry buffering the transcriptional network directing muscle development.

2015-04-01 | GSE28605 | GEO

Ectopic expression of mir-3 affects mef2 and tinman levels

Project description:Loss of nautilus (MyoD) gene function results in a variable phenotype affecting muscle formation in embryos and larvae, larval movement, pupal eclosion, egg deposition, adult mobility and survival. mir-3 over expression disrupts muscle formation in the embryo while affecting protein production from the dMef2 and tinman genes, global regulators of the muscle transcriptional network. We propose the complex phenotype in the nautilus null is due to the disruption of the regulatory interactions provided by the 8-miR cluster. The results demonstrate that nautilus is an integral regulator of the miRNA circuitry buffering the transcriptional network directing muscle development. Two-condition experiment, wild type (w1118) vs. mutant (mir-3 ectopic expressionl). Biological replicates: 3 wild type, 3 mutants, independently isolated. One of the biological replicate was dye swaped to avoid dye bias.

2015-04-01 | E-GEOD-28605 | biostudies-arrayexpress

Drosophila melanogaster

Project description:nautilus null vs. wild type miRNA

| PRJNA143035 | ENA

Hi-C in Drosophila embryos at NC14 depleted of Beaf-32, CP190 or CTCF using the Bridge Adaptor Protocol

Project description:Hi-C was performed using the Bridge Adaptor Protocol in 2 biological replicates of Drosophila embryos at NC14 depleted of the maternal deposition of Beaf-32, CP190 or CTCF mRNA and protein. Embryos were collected at 2-3h AEL and were derived from mothers expressing RNAi against Beaf-32 or CP190 in the germline, or were genetic null for the maternal and zygotic contribution of CTCF.

2022-12-17 | E-MTAB-9158 | biostudies-arrayexpress

A genome-wide analysis reveals that the Drosophila transcription factor, Lola, promotes axon growth in part by suppressing expression of the actin nucleation factor, Spire (allele lola_ORE76)

Project description:Transcriptional profiling of Drosophila embryos comparing lola mutant 10-12 hour after egg lay (AEL) embryos to age-matched control embryos. Mutants are homozygous lola nulls (allele lola_ORE76).

2011-12-03 | GSE34121 | GEO

A genome-wide analysis reveals that the Drosophila transcription factor, Lola, promotes axon growth in part by suppressing expression of the actin nucleation factor, Spire (allele lola_ORC4)

2011-12-03 | GSE34122 | GEO

Dm miR-309 cluster KO Expression Profiling

Project description:To investigate whether early zygotic miR-309 cluster miRNA expression might contribute to the down-regulation of maternal transcripts during the maternal to zygotic transition in Drosophila, we performed microarray analyses of control and miR-309 cluster mutant embryos at 0-1h and 2-3h of embryonic development. One-hour egg collections of w1118 and miR-309-6delta1 mutant flies at 25?C were either aged (2-3h sample) at 25?C or directly processed (0-1h sample). Six independent collections per genotype and time-point were performed. Using this approach, we found that maternal transcripts were stabilized in the miR-309 cluster mutant, indicating that the miR-309 cluster miRNAs are required for the down-regulation of these transcripts during the maternal to zygotic transition in Drosophila.

2009-01-27 | E-TABM-441 | biostudies-arrayexpress

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