Project description:To investigate the dynamics of gene expression during T cell activation and identify essential genes responsive to stimulation, we conducted RNA sequencing on both resting and stimulated Jurkat cells.
Project description:The majority of genetic risk variants associated with autoimmune diseases are located in chromatin regions that are accessible during T cell stimulation. To systematically investigate the dynamic changes in chromatin accessibility during T cell activation, we utilized the Jurkat cell line as a model and performed ATAC-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells.
Project description:To finely dissect the dynamic changes in the epigenome and chromatin state during T cell activation, we utilized the Jurkat cell line as a model and performed ChIP-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells, targeting various histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), RNA PolII, and CTCF.
Project description:To investigate the interacting loci within the region containing rs5837875, we employed the circularized chromosome conformation capture (4C) assay to elucidate the spatial interactions and regulatory networks associated with rs5837875, utilizing this specific region as the bait in Jurkat cells.
Project description:To meticulously explore how local chromatin architecture influences T cell activation, we conducted high-resolution regional capture Hi-C by designing tiling capture probes that span the CD28, CTLA4, and ICOS genes along with their distal regulatory sequences (chr2:204300000-205000000, GRCh37) in both resting and stimulated Jurkat cells.
