Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nascent RNA m6A modification are involved in T cell activation gene signature under Hypoxia in CRPC-V16A

ABSTRACT: In this study, we set out to identify m6A modified nascent transcription under Hypoxia in parallel with Normoxia by EU labeling coupled with MeRIP-seq. By comparing Hypoxia and Nomoxia, we identified 5,873 m6A sites specific under Hypoxia.

ORGANISM(S): Homo sapiens

PROVIDER: GSE285894 | GEO | 2025/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

m6A modification profile in primary microglia with/without Igf2bp1 knockdown

Project description:Our study demonstrated that the expression of Igf2bp1 in activated microglia was significantly up-regulated, implying a role of Igf2bp1 in LPS-induced m6A modifications in microglia. To understand the roles of Igf2bp1 on LPS-induced m6A modification in microglia, we performed Igf2bp1 loss-of-function (LOF) approach. Microglia stimulated by LPS were transfected with either scrambled siRNA control or Igf2bp1 siRNA for 48 hours. To m6A modification profiles in control and Igf2bp1 LOF microglia were determined by MeRIP-seq analysis.

2022-12-31 | E-MTAB-11411 | biostudies-arrayexpress

m6A epitranscriptomic changes in response to hypoxic condition or ALKBH5 knock-down in U87

Project description:Compared with the normoxia control, 174 m6A peaks from 58 m6A-modified transcripts are significantly increased while 2032 m6A peaks from 556 m6A-modified transcripts are significantly decreased in hypoxia. Among the downregulated m6A peaks in hypoxia compared with normoxia condition, ALKBH5 depletion leads to 847 increased m6A peaks in 276 transcripts (fold change≥1.5).

2024-03-31 | GSE171497 | GEO

Nascent RNA m6A modification are involved in T cell activation gene signature under Hypoxia in CRPC-V16A

Project description:Nascent RNA m6A modification are involved in T cell activation gene signature under Hypoxia in CRPC-V16A

| PRJNA1206721 | ENA

MeRIP sequencing for CSPC vs CRPC samples

Project description:To investigate if there is a difference of N6-methyladenosine(m6A) modification between castration-resistant prostate cancer (CRPC) and castration-sensitive prostate cancer (CSPC), we collected 30 specimens, including 15 CRPC and 15 CSPC, to perform RNA-seq and MeRIP-seq. All specimens were postoperative tissues and each 5 CRPC or CSPC specimens were mixed into 1 sample to meet the RNA dosage of RNA-seq and MeRIP-seq.

2023-01-03 | GSE221961 | GEO

Impact of gut microbiota on m6A/m epitranscriptomic mRNA modification in different host tissues

Project description:In this study we performed MeRIP-Seq to study N6-methyl adenosine (m6A) and and N6,2′ -O-dimethyladenosine (m6Am) modification of mRNA. We investigated the effect of the microbiota on the transcriptome and epitranscriptomic modifications in murine liver and cecum. We compared m6A/m modification profiles in cecum of conventionally raised (CONV) and germ-free (GF) mice. We additionally included GF mice colonised with the flora of CONV mice for four weeks (ex-GF), for which show that they exhibit similar patterns of the most abundant genera of gut bacteria as CONV mice. We added mice treated with several antibiotics to deplete the gut flora (abx)and vancomycin treated mice in which the genera Akkermansia, Escherichia/Shigella and Lactobacillus were enriched. Furthermore, we included GF mice colonised with the commensal bacterium Akkermansia muciniphila (Am), Lactobacillus plantarum (Lp) and Escherichia coli Nissle (Ec) and analysed their m6A/m modification profiles. In addition, we analysed changes in m6A/m- modified liver RNA for CONV, GF, and Am, Lp and Ec mice.

2020-02-12 | E-MTAB-6560 | biostudies-arrayexpress

Identify mRNA with m6A modification in PLC-8024 cells [MeRIP-seq]

Project description:To investigate the m6A mapping in HCC, we employed MeRIP-seq to identify mRNAs with m6A modification

2023-02-28 | GSE206503 | GEO

Mycobacterium tuberculosis normoxia hypoxia reactivation with ActivX-desthiobiotin FP probe

Project description:Lysates of Mycobacterium tuberculosis (H37Rv auxotroph mc(2)6020) grown under various conditions (normoxia, hypoxia, reactivation from hypoxia) probed the serine hydrolase probe with ActivX-desthiobiotin FP.

2016-10-10 | MSV000080238 | MassIVE

Hypoxia and lymphatic endothelial cells

Project description:Lymphatic endothelial cells were grown under normoxia, hypoxia (1% 0xygen) and conditioned medio from NSLCN growth under normoxia or hypoxia. Gene expression was measured and comparition between samples performed Keywords: Comparative genomic hybridization

2007-08-23 | GSE5579 | GEO

Dictyostelium discoideum whole cell proteome from AX3 (WT) vegetative cells under hypoxia or normoxia 5h incubation time

Project description:Dictyostelium discoideum whole cell proteome from WT (AX3) vegetative cells under hypoxia were analyzed at 0h (before any treatment) and 5h after hypoxia or normoxia treatment. The experiment consists of 6 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 at 0h against AX3 at 5h under hypoxia or normoxia incubations.

2025-11-04 | PXD061993 | Pride

FTO suppresses cardiac fibrosis after myocardial infarction via m6A-mediated epigenetic modification

Project description:Cardiac fibrosis is common in cardiovascular diseases. N6-methyladenosine (m6A) is one of the most common modifications in eukaryotic mRNAs. Previous research has suggested that m6A modification is vital in cardiovascular diseases. The underlying targets of FTO were selected through transcriptome sequencing (RNA-seq) combined with methylated RNA immunoprecipitation sequencing (MeRIP-seq). According to MeRIP-seq and RNA-seq, FTO inhibited collagen synthesis in CFs.

2024-03-01 | GSE257546 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data