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Low-potassium triggers epithelial NLRP3/ASC-driven kidney inflammation independently of inflammasomes

ABSTRACT: We have employed a single cell RNA sequencing analysis using 10x genomics scRNAseq to study the effect of potassium deficiency on the non-leukocyte kidney cells. Long-term potassium deficiency leads to kidney injury and inflammation. This study investigated a pro-inflammatory cascade in the kidney epithelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE286080 | GEO | 2025/08/14

REPOSITORIES: GEO

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Low-potassium triggers epithelial NLRP3/ASC-driven kidney inflammation independently of inflammasomes

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Project description:Inflammasomes are protein complexes assembled upon recognition of infection or cell damage signals, and serve as platforms for clustering and activation of procaspase-1. Oligomerisation of initiating proteins such as AIM2 (absent in melanoma-2) and NLRP3 (NOD-like receptor family, pyrin domain-containing-3) recruits procaspase-1 via the inflammasome adapter molecule ASC (apoptosis-associated speck-like protein containing a CARD). Active caspase-1 is responsible for rapid lytic cell death termed pyroptosis. Here we show that AIM2 and NLRP3 inflammasomes activate caspase-8 and -1, leading to both apoptotic and pyroptotic cell death. The AIM2 inflammasome is activated by cytosolic DNA. The balance between pyroptosis and apoptosis depended upon the amount of DNA, with apoptosis seen at lower transfected DNA concentrations. Pyroptosis had a higher threshold for activation, and dominated at high DNA concentrations because it happens more rapidly. Gene knockdown showed caspase-8 to be the apical caspase in the AIM2- and NLRP3-dependent apoptotic pathways, with little or no requirement for caspase-9. Procaspase-8 localised to ASC inflammasome 'specks' in cells, and bound directly to the pyrin domain of ASC. Thus caspase-8 is an integral part of the inflammasome, and this extends the relevance of the inflammasome to cell types that do not express caspase-1.

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Project description: Not available

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Effect of potassium-depleted diet on kidney

Project description:Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other

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De novo characterization of alligator weed (Alternanthera philoxeroides) transcriptome shedding light on gene expression under potassium deprivation

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