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Integrated network pharmacology and transcriptomics to explore the mechanism of Gui-qi-yi-shen granules in the treatment of IgA nephropathy

ABSTRACT: To elucidate the underlying therapeutic mechanisms of Gui-qi-yi-shen granules (GQYS) in the treatment of IgAN, we have employed transcriptome sequencing of 9 mice kidney samples. 155 differentially genes were identified between IgAN and control groups, with 125 upregulated and 30 downregulated. Meanwhile, 403 genes differed between GQYS and IgAN groups, including 171 upregulated and 332 downregulated genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE286126 | GEO | 2025/05/01

REPOSITORIES: GEO

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Project description:Background Also the traditional Chinese medicine (TCM) theory of "Yang transforming into Qi with Yin constituting Form" was recognized as a potential mechanism underlying the tumorigenesis. The decoction based on this theory and the underlying molecular mechanisms remained exploration. Methods A TCM decoction was prepared based on "Yang transforming into Qi with Yin constituting Form". Its anti-tumorigenesis effect was evaluated on mouse models of hepatocellular carcinoma (HCC) (H-rasG12V transgenic mice (Ras-Tg) and Diethylnitrosamine (DEN)-induced HCC mice) by liver/body weight, number and size of tumors, histopathological changes and serum biochemistry. RNA sequencing was utilized to check differentially expressed genes (DEGs) and gene ontology (GO) and KEGG were used for bioinformatics analysis. The concerned biological processes were further confirmed by RT-qPCR and metabolite detection kits. Results The TCM decoction significantly suppressed both Ras oncogene- and DEN-induced hepatic tumorigenesis evidenced by reduced serum ALT and AST levels, liver weight, liver/body weight ratio, hepatic tumor numbers and sizes. RNA sequencing showed 659 up-regulated genes and 222 down-regulated genes in comparison of liver tissues between TCM-treated and untreated Rag-Tg (n=5). GO entries and KEGG analysis revealed multiple biological processes and pathways involving the hepatic tumorigenesis suppressing effective of the TCM. Further molecular examination confirmed that activation of retinal metabolism, reduction of ROS and electrophilic levels by inhibiting the H2O2 production and promoting GSH production, and inhibition of expression of inflammation factors play pivotal roles in the anti-tumorigenesis effects of TCM. Conclusion A TCM decoction based on "Yang transforming into Qi with Yin constituting Form" showed effective anti-hepatic tumorigenesis functions and its regulation functions on retinal, ROS and inflammation processes play important roles. The present study provides valuable clue for clinical therapy on tumorigenesis.

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Integrated network pharmacology and transcriptomics to explore the mechanism of Gui-qi-yi-shen granules in the treatment of IgA nephropathy

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