Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

High-throughput unbiased RNA-Sequencing (RNA-Seq) to profile gene expression in ipsilateral L4-L6 DRG obtained from rats of Sham+Veh, CPIP+Veh and CPIP+ETA groups

ABSTRACT: Since TNF-α is a pro-inflammatory cytokine that can promote neuroinflammation to mediate chronic pain, we then explored which signaling molecules or pathways in DRG were affected by TNF-α and contributed to neuroinflammation in CPIP condition.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE286312 | GEO | 2025/04/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell RNA seq of ipsilateral L4-L6 DRGs from rats in both Sham and CPIP group

Project description:Complex regional pain syndrome type-I (CRPS-I) is chronic neurological disorder accompanied with devastating pain. In this study, we employed single-cell sequencing technology to analyze the ipsilateral L4-L6 dorsal root ganglia in both Sham and CPIP groups, aiming to investigate the mechanisms underlying chronic pain development in the CPIP model.

2025-04-14 | GSE294564 | GEO

High-throughput unbiased RNA-Sequencing (RNA-Seq) to profile gene expression in ipsilateral L4-L6 DRG obtained from rats of Sham+Veh, CPIP+Veh and CPIP+ETA groups

Project description:High-throughput unbiased RNA-Sequencing (RNA-Seq) to profile gene expression in ipsilateral L4-L6 DRG obtained from rats of Sham+Veh, CPIP+Veh and CPIP+ETA groups

| PRJNA1208658 | ENA

Single-cell RNA seq of ipsilateral L4-L6 DRGs from rats in both Sham and CPIP group

Project description:Single-cell RNA seq of ipsilateral L4-L6 DRGs from rats in both Sham and CPIP group

| PRJNA1250505 | ENA

Off-target effect of CWG-cPIP on gene expression of human fibroblasts

Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in cells, CWG-cPIP (1 μM) was treated to human fibroblasts. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-treated human fibroblasts 7 days later, mixing with ERCC RNA spike-in controls.

2023-10-11 | GSE235810 | GEO

Off-target effect of CWG-cPIP on gene expression of mouse Striatum

Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in the brain, CWG-cPIP (664 ug/kg) was intracerebroventricularly injected into mouse. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-injected mouse striatum 3 weeks later, mixing with ERCC RNA spike-in controls.

2023-10-11 | GSE235804 | GEO

Off-target effect of CWG-cPIP on gene expression of mouse hippocampus

Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate off-target effects of CWG-cPIP on gene expression in the brain, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using vehicle- or CWG-cPIP-injected mouse hippocampus 3 weeks later, mixing with ERCC RNA spike-in controls.

2023-10-11 | GSE235801 | GEO

Effect of CWG-cPIP on transcriptome defects in CUG mouse brain

Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate effects of CWG-cPIP on transcriptome defects in the brain of CUG300 mice, which we developed as a model of DM1, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using mouse hippocampus 10 days later.

2023-10-11 | GSE210838 | GEO

Effect of CWG-cPIP on alternative splicing defects in CUG mouse brain

Project description:CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate effects of CWG-cPIP on alternative splicing defects in the brain of CUG300 mice, which we developed as a model of DM1, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using mouse hippocampus 10 days later.

2023-10-11 | GSE210837 | GEO

Stress prevalence in a normal Thai population and its relationship with MDD-related point mutations, HHV-6 infection, and TNF-α

Project description:This project analyzes the correlation between stress prevalence in a normal Thai population and MDD-related point mutations, HHV-6 infection, and TNF-α levels. The study aims to reveal molecular insights into stress-induced neuroinflammation and genetic susceptibility to depression.

2025-06-06 | PXD064692 | JPOST Repository

Inhibition of tumour necrosis factor alpha by Etanercept attenuates Shiga toxin-induced brain pathology

Project description:Infection with enterohemorrhagic E. coli (EHEC) causes severe changes in the brain leading to angiopathy, encephalopathy and microglial activation. In this study, we investigated the role of tumour necrosis factor alpha (TNF-α) for microglial activation and brain pathology using a preclinical mouse model of EHEC infection. LC–MS/MS proteomics of mice injected with a combination of Shiga toxin (Stx) and lipopolysaccharide (LPS) revealed extensive alterations of the brain proteome, in particular enrichment of pathways involved in complement activation and coagulation cascades. Inhibition of TNF-α by the drug Etanercept strongly mitigated these changes, particularly within the complement pathway, suggesting TNF-α-dependent vasodilation and endothelial injury. Analysis of microglial populations using a novel human-in-the-loop deep learning algorithm for the segmentation of microscopic imaging data indicated specific morphological changes, which were reduced to healthy condition after inhibition of TNF-α. Moreover, the Stx/LPS-mediated angiopathy was significantly attenuated by inhibition of TNF-α. Overall, our findings elucidate the critical role of TNF-α in EHEC-induced brain pathology and highlight a potential therapeutic target for mitigating neuroinflammation, microglial activation and injury associated with EHEC infection.

2025-02-18 | PXD052925 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data