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Microtopography-induced changes in cell nucleus morphology enhance bone regeneration by modulating the cellular secretome

ABSTRACT: To investigate the influence of micropillar implants on bone regeneration, we created two critical size cranial wound repair model using implants with flat and micropillar surface topography

ORGANISM(S): Mus musculus

PROVIDER: GSE286676 | GEO | 2025/05/01

REPOSITORIES: GEO

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Micropillar-induced changes in cell nucleus morphology enhance bone regeneration by modulating the secretome

Project description:To investigate the influence of micropillars on proteomics, we seeded cells on flat and micropillar surfaces followed by (1) collection of proteins in cells on flat and micropillar surfaces; (2) collection of proteins secreted into medium by cells on flat and micropillar surfaces.

2025-05-19 | PXD059752 | Pride

Microtopography-induced changes in cell nucleus morphology enhance bone regeneration by modulating the cellular secretome

Project description:Microtopography-induced changes in cell nucleus morphology enhance bone regeneration by modulating the cellular secretome

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Early molecular assessment of osseointegration in humans

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Early molecular assessment of osseointegration in humans

Project description:To determine the early temporal wide genome transcription regulation by the surface topography at the bone-implant interface of implants bearing micro-roughened or superimposed nanosurface topology. Fourty four cp titanium implants with surface topographies exhibiting nanoscale features (Osseospeed-OS) and microrough surface without nanoscale features (TiOblast-TiO) were placed in the alveolar bone of 11 systemically healthy subjects and subsequently harvested at 3 and 7 days after placement. Total RNA was isolated from cells adherent to retrieved implants. A whole genome microarray using the Affymetrix Human gene 1.1 ST Array was used to describe the gene expression profiles that were differentially regulated by the implant surfaces.

2013-09-17 | E-GEOD-41446 | biostudies-arrayexpress

Single-cell Analysis of Surface Topography-induced Foreign Body Response to Silicone Implants

Project description:Silicone-based medical devices are widely used in chronic implants and are generally perceived to be safe. However, immune-related complications including malignancies have recently been linked to textured breast implants. Here, we examine the influence of clinically approved breast implants surface features on host immune responses. Prosthetics with surface roughness of 0, 4, and 90 (Ra) were implanted in mammary fat pads of mice for 2 weeks and cells adjacent to the resulting tissue capsules were evaluated for foreign body immune responses using single-cell RNA-seq. Our findings identify a unique and finely tuned surface topography that is capable of modulating implant immunity to suppress foreign body response.

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Chromatin site specific accessibility: a microtopography regulated door into the stem cell fate [Hi-C]

Project description:Biomaterials engineered to mimic extracellular matrix (ECM) topography play a pivotal role in tissue engineering. Previous research indicates that certain biomimetic topographies can guide stem cells towards multiple specific lineages. However, the mechanisms by which these topographic cues prime the multilineage differentiation remain largely elusive. In this study, we show that topography influences nuclear tension in MSCs through a mechanotransductive feedback mechanism, which in turn reshapes chromatin accessibility patterns, finally determines the stem cell lineage commitment. When cultured on an aligned substrate, MSCs show high cytoskeletal tension along the fibre direction, resulting in anisotropic nucleus tensile stress. This further leads to the opening of chromatin sites related to neurogenic, myogenic, and tenogenic genes by the regulation of transcription factors such as TLX. On the other hand, when MSCs are lied on a random substrate, they experience isotropic nucleus stress, leading to the opening of chromatin sites related to osteogenic and chondrogenic genes through the regulation of RUNX family transcription factors. Collectively, our results show that aligned and random topography induce site specific chromatin stretching and lineage specific gene expressing in MSCs, leading to their priming for specific lineages. Our study thus proposes a novel concept for topographic cues influencing on cell behavior and fate during tissue reconstitution and regeneration.

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Chromatin site specific accessibility: a microtopography regulated door into the stem cell fate [ATAC-seq]

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Investigation of titanium discs produced by Straumann on human mesenchymal stromal cells

Project description:In this study the gene expression differences between three titanium surfaces produced by Straumann were investigated. These three surfaces were: flat pre-treated (Pt) titanium, sandblasted (S) titanium and sandblasted acid-etched (SLA) titanium. The SLA surface is known to boost the proliferation and osteogenic differentiation of MG-63 cells. SLA titanium is also widely used for dental implants.

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Novel integrated multiomics analysis reveals Itgbl1-mediated regulation of fibrogenesis through antagonism of Tgfβ1 and IL1β signaling. (Spatial Gene Expression)

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Nanotopography guides morphology and spatial patterning of induced pluripotent stem cell colonies

Project description:The role of topographic cues in controlling commitment of induced pluripotent stem cells (iPSCs) is largely unknown. Here we demonstrate that groove-ridge nanostructures induce the elongation of iPSC colonies, guide the orientation of apical actin fibers and direct the polarity of cell division. Elongation of iPSC colonies impacts also on the intrinsic molecular patterning which seems to be orchestrated starting from the rim of the colonies. We followed the hypothesis that nanotopography directly modulates the transcriptional program of iPSC, further to guiding the overall spatial organization of the colonies. Single iPSC were seeded on flat (PI flat) and nanostructured polyimide (PI 650) and gene expression profiles were analyzed after three days. No significant differences were observed when cells were kept under culture conditions that sustained pluripotency. Then, we analyzed gene expression changes upon two weeks of multi-lineage differentiation. Many genes revealed significant expression changes in the course of differentiation and this was more pronounced on PI flat as compared to PI 650. Comparison of iPSC that were either differentiated on flat or nanostructured biomaterials revealed differential expression of several genes. Noteworthy, among significantly regulated genes, the biggest fold change on PI 650 versus PI flat after differentiation was observed in ANKRD1, which is one of the best readouts of YAP/TAZ activity. Our study suggests that nanotopography impacts on orientation and organization of iPSC colonies and highlight a possible interaction between mechanosensors and mechanotransducers.

2017-09-18 | GSE84848 | GEO

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