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Gene expression profiling of epithelial and mesenchymal subpopulations within immortalized human mammary epithelial cells

ABSTRACT: For gene expression profiling, we used immortalized human mammary epithelial cells (HMLE) to isolate a pure epithelial fraction of cells by positive selection for CD24 expression using Magnetic Activated Cell Sorting (=24hi). We independently isolated 3 mesenchymal subpopulations (msp1-3) from HMLE cells by collecting floating cells from cultured monolayer HMLE cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE28681 | GEO | 2011/04/19

SECONDARY ACCESSION(S): PRJNA138939

REPOSITORIES: GEO

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Gene expression profiling of epithelial and mesenchymal subpopulations within immortalized human mammary epithelial cells

Project description:For gene expression profiling, we used immortalized human mammary epithelial cells (HMLE) to isolate a pure epithelial fraction of cells by positive selection for CD24 expression using Magnetic Activated Cell Sorting (=24hi). We independently isolated 3 mesenchymal subpopulations (msp1-3) from HMLE cells by collecting floating cells from cultured monolayer HMLE cells. See summary

2011-04-19 | E-GEOD-28681 | biostudies-arrayexpress

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Differences between CD44+/CD24- and CD44-/CD24+ subpopulation of immortalized human mammary epithelial cells

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Systems level analysis of epithelial to mesenchymal transition reveals more alteration in phosphoproteome than proteome and identifies DNAJB4 and CD81 as EMT regulators

Project description:We quantified protein expression changes between epithelial and mesechymal stages in immortalized human mammary epithelial cells (HMLE). Epithelial–mesenchymal transition is induced by expressing an EMT-TF, Twist.

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