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Measuring DNA replication timing of maize root tips using DNA copy number (S/G1) method

ABSTRACT: During the cell cycle, DNA replication is a highly regulated process that integrates the time that a genome segment replicates in S phase with its transcriptional activity, chromatin structure, and epigenetic state. We are using fluorescence activated nuclei sorting in combination with high throughput sequencing to characterize the patterns of DNA replication across the maize B73 genome using a DNA copy number based procedure.

ORGANISM(S): Zea mays

PROVIDER: GSE287085 | GEO | 2025/04/29

REPOSITORIES: GEO

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Measuring DNA replication timing of maize root tips using DNA copy number (S/G1) method with an EdU lableing step for bivariate sorting of S and G1 phase

Project description:DNA replication is a highly regulated cell cycle process that integrates the time that a genome segment replicates in S phase with its transcriptional activity, chromatin structure, and epigenetic state. We use both DAPI and 5-ethynyl-2'-deoxyuridine (EdU) labeling to perform fluorescence activated nuclei sorting (FANS) in combination with high throughput sequencing to characterize the patterns of DNA replication across the maize B73 genome using a DNA copy number based procedure.

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Measuring DNA replication timing of maize root tips with Repli-seq method

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Fused in sarcoma regulates DNA replication timing and progression

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Project description:Human DNA Replication Timing Variation

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