Transcriptomics

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Human Immuno-Lung Organoid Model to Study Macrophage-Mediated Lung Cell Senescence Upon SARS-CoV-2 Infection [scRNA-seq]


ABSTRACT: In this study, we utilized spatial transcriptional assays (NanoString CosMx) to analyze both explant and autopsy samples from COVID and non-COVID-19 lungs, identifying activation of proinflammatory macrophages. We then created macrophage-alveolar and macrophage-airway organoid models, integrating hPSC-derived macrophages with alveolar or airway organoids, and discovered that proinflammatory macrophages induce lung cell senescence through via the THBS1-(ITGA3+ITGB1) pathway. This study not only establishes immune-lung organoid models for studying macrophage-mediated host damage but also highlights the previously unrecognized role of the THBS1-(ITGA3+ITGB1) pathway in lung cell senescence during infectious diseases.

ORGANISM(S): Homo sapiens

PROVIDER: GSE287111 | GEO | 2025/11/14

REPOSITORIES: GEO

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