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Cell-free DNA Methylomics Identify Tissue Injury Patterns in Pediatric ARDS

ABSTRACT: Cell-free DNA (cfDNA) is released into the circulation from dead and dying cells, and is elevated in critical illness syndromes including sepsis and acute respiratory distress syndrome (ARDS). Plasma cfDNA has been implicated as a damage-associated molecular pattern (DAMP) that propagates ongoing inflammation. However, the tissue source of circulating cfDNA from different pathological processes has not been fully elucidated, and identification of the cellular origin of cfDNA may provide insight into the mechanisms underlying the early inflammatory response. As cfDNA retains the unique methylation pattern of its tissue of origin, we can leverage cfDNA methylome to map the presumed tissue of origin of these DAMPs. We conducted a pilot study to assess the cfDNA methylation pattern in pediatric ARDS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE287137 | GEO | 2025/08/15

REPOSITORIES: GEO

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