Single-cell and single-nucleus transcriptome analysis of stripe pattern formation in the embryo of the spider Parasteatoda tepidariorum

ABSTRACT: Segmented body plans are a hallmark of arthropods, yet their underlying developmental processes vary widely. We applied single-cell and single-nucleus RNA sequencing of Parasteatoda tepidariorum embryos at late stage 5 and stage 7, a critical period of emergence of segmental units along the anterior-posterior (AP) axis. Single-nucleus RNA sequencing yielded high-quality quantitative data, and subsequent clustering analysis reconstructed an axial alignment of ectoderm cells, reflecting the differing cell states along the segmenting AP axis. This enabled us to generate genome-wide quantitative gene expression profiles along the reconstructed axis. Employing unbiassed statistical methods, we comprehensively identified pattern elements. These data lay the groundwork for exploring the origins of developmental diversity for arthropod body plans.

ORGANISM(S): Parasteatoda tepidariorum

PROVIDER: GSE287446 | GEO | 2025/05/31

REPOSITORIES: GEO