Aging and the pathophysiology of pulmonary Mycobacterium avium complex (MAC) disease
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE287501 | GEO | 2026/03/18
REPOSITORIES: GEO
Similar Datasets
Project description:We focused on how Mycobacterium avium subsp. paratuberculosis influences the subsequent host response to investigate the host immunopathology accompanying the host anti-mycobacterial immune response during Mycobacterium avium subsp. paratuberculosis infection in spleen of mice.
2014-10-31 | GSE62836 | GEO
Project description:We focused on how Mycobacterium avium subsp. paratuberculosis influences the subsequent host response to investigate the host immunopathology accompanying the host anti-mycobacterial immune response during Mycobacterium avium subsp. paratuberculosis infection in spleen of mice. We analyzed altered transcription in the spleen of mice at 3, 6, and 12 weeks following Mycobacterium avium subsp. paratuberculosis infection.
2014-10-31 | E-GEOD-62836 | biostudies-arrayexpress
Project description:Aging and the pathophysiology of pulmonary Mycobacterium avium complex (MAC) disease
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Project description:WGS of MAC strains from infected BALB/c mice
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Project description:To investigate the changes in global transcriptome expression of HSCs following infection, we performed high-throughput sequencing of Poly A+ RNA (RNA-Seq) from purified HSCs (SP KSL CD150+) isolated from naive or Mycobacterium avium infected mice
2017-01-04 | GSE89364 | GEO
Project description:Mycobacterium avium subspecies paratuberculosis (MAP) infection is established following the ingestion of bacteria, their penetration of the intestinal mucosa and subsequent events of the host-parasite interaction. These bacteria invade M cells, macrophages and are capable of resisting host defenses and multiply to reach very high numbers intracellular. Mycobactrium avium subsp. avium (MAA) is an antigenically and genetically similar organisms but is relatively nonpathogenic for cattle. MAA organisms appear to infect cattle, but unlike cattle infected with MAP, cattle infected with MAA typically mount an effective systemic immune response, form caseous granuloma, and eliminate the infection. This study was designed to understand the similarities and differences in the host responses during MAP and MAA infection. Neonatal calves were infected (ligated ileal loops) with PBS, MAP, or avirulent MAA following which samples were collected at 30 min, 1h, 2h, 4h, 8h, or 12h. Post-infection, RNA was collected, processed, and then hybridized to custom bovine gene expression arrays, each of which represented 13,258 transcripts spotted in duplicate. Arrays were normalized by scaling against the average reference intensity value (i.e., average across all arrays), normalized by global mean, and then log transformed before statistical analyses were performed. Pairwise comparisons of averaged signal values and Student’s t test were performed using GeneSifter software (VizX Labs, Seattle, WA). A fold-change of at least 1.5-fold and a p value of 0.05 or less was required for a difference in signal to be considered statistically significant. When comparing the transcriptional responses of calves infected with the MAA versus MAP, unique patterns of expression were clearly visible. In general, numbers of transcripts altered in response to infection were much greater for MAA-infected animals than for those infected with the MAP. No genes were significantly up-regulated in MAP-infected animals at the earliest time point tested (30 min), and only modest numbers of genes were increased in expression over the experimental time course. On the other hand, up-regulated transcripts were detected by 30 min in MAA-infected animals and peaked by 2 hr. Differences in the numbers of down-regulated genes between MAP-infected and MAA-infected animals (compared to PBS-infected controls) were less pronounced. At the earliest time points, MAP-infected calves had a greater number of down-regulated genes than did animals infected with MAA. This trend was reversed at 8 and 12 hr post-infection. Keywords: Gene expression profiling by microarray
2012-10-03 | GSE13888 | GEO
Project description:Once entering the cell, M. avium subsp.paratuberculosis is known to survive harsh microenvironments, especially those inside activated macrophages. To improve our understanding of M. avium subsp.paratuberculosis pathogenesis, we examined the phagosome maturation associated with transcriptional responses of M. avium subsp.paratuberculosis during macrophage infection. Monitoring cellular markers, only live M. avium subsp.paratuberculosis bacilli were able to prevent phagosome maturation and reduce its acidification. On the transcriptional level, over 300 of M. avium subsp.paratuberculosis genes were significantly, differentially regulated in both naïve and IFN-γ-activated macrophages. These genes include the sigma factor H (sigH) that was shown to be important during persistent infection in M. tuberculosis.
2013-01-22 | GSE43645 | GEO
Project description:Crohn’s disease (CD) is a chronic inflammatory bowel disease (IBD) in humans. Mycobacterium avium, which causes Johne’s disease in ruminants, has been suggested as a potential CD trigger due to similar pathology. However, early epithelial responses to M. avium and their relevance to CD remain unclear. This study aimed to establish a mouse small intestinal organoid (mSIO) model of M. avium infection and assess whether the resulting epithelial and immune responses resemble CD-related inflammation. M. avium-infected mSIOs were analyzed using confocal microscopy, serial block-face scanning electron microscopy, and a macrophage co-culture system to observe bacterial localization and immune cell behavior. RNA-seq and Gene Set Enrichment Analysis (GSEA) were performed to assess transcriptional changes. Comparative transcriptomic analysis was performed against a previous published RNA-seq dataset from gut tissues of germ-free mice colonized with microbiota from CD patients. M. avium efficiently infected mSIOs, disseminated through the tissue and triggered macrophage migration and clustering around infected organoids. Transcriptomic profiling revealed strong upregulation of inflammatory genes and enrichment of pro-inflammatory pathways such as TNF-α signaling. Comparative analysis revealed significant overlap in gene expression profile between M. avium-infected mSIOs and intestinal tissues of CD-humanized mice, including shared enrichment of the IBD signaling pathway. Notably, Mmp7, involved in epithelial remodeling and inflammation, emerged as a common marker in both models. This study presents a robust mSIO model of M. avium infection that replicates key features of CD-associated inflammation. The model presents a useful tool for studying host-pathogen interactions and identifies Mmp7 as a molecular link between bacterial infection and CD-like pathology.
2025-09-01 | GSE305886 | GEO
Project description:Mycobacterium avium is one of the prominent disease causing bacteria in humans. It causes lymphadenitis, chronic pulmonary and extrapulmonary and disseminated infections in adults, children and immunocompromised humans. M. avium has ~4,500 predicted gene models, out of which not all are identified at proteomic level. Proteomic database search followed by proteogenomic analysis helps in the correction of gene models, identification of novel exons/genes, variant proteins. As part of this study, we performed proteomic analysis of M. avium cultures by data-dependent acquisition (DDA) and data-independent acquisition (DIA) method followed by proteogenomic analysis of M. avium proteomic data. M. avium culture was subjected to proteomic sample preparation. The resulting peptides were acquired in 120min DDA (12 bRPLC) and DIA method using EASY nLC 1200 liquid chromatogram system coupled to Orbitrap Fusion Tribrid mass spectrometer. The resulting DDA raw files were searched sequentially against the M. avium proteome database, Mycobacterium tuberculosis H37Rv and Ra proteome database (Mtb), M. avium genome six-frame translated proteome and variant proteins database, respectively. The database search result was converted into a spectral library and used for DIA data search for the validation of GSSPs and variant peptides. The database search of M. avium DDA has resulted in the identification of 2,954 M. avium proteins, 128 Mtb proteins, 174 GSSPs (M. avium genome six-frame translated proteome) and 795 SNPs corresponding to 612 proteins (variant proteins database), respectively. The M. avium proteome database search has covered 62.09% of the proteome with the identification of 2,954 proteins out of 4,757 proteins in the database. From the manual categorization of 174 GSSPs, we observed 23 N-terminal extensions, 142 pseudogene coding peptides and one each novel exon and short peptide, respectively.
2023-03-17 | PXD032067 | Pride