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Gene expression analysis of HepG2 cells overexpressing TAF2

ABSTRACT: Taf2 is a transcription co-factor and a member of TFIID complex. To better understand gene regulation of Taf2 we generated stable clones of HepG2 cells overexpressing either eGFP or TAF2-V5. The analysis shows that overexpression of TAF2 activates several pro-tumorigenic pathways and inhibits tumor suppressor pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE287510 | GEO | 2025/12/12

REPOSITORIES: GEO

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ChIP-seq analysis in HepG2-TAF2-V5 clone

Project description:Taf2 is a transcription co-factor and a member of TFIID complex. To better understand gene regulation by Taf2 we generated HepG2 cells stably overexpressing TAF2-V5. This clone was used for ChIP using control IgG or V5 antibody. We identified TAF2-V5 binding near the transcription start sites of 37 genes.

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Gene expression analysis of HepG2 cells overexpressing TAF2

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TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing factors [CUT&RUN]

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Taf2 mediates DNA binding of Taf14

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