Transcriptomics

Dataset Information

0

Simultaneous profiling of native-state proteomes and transcriptomes of brain cell types using proximity labeling


ABSTRACT: Phenotyping cells at transcriptomic and proteomic levels is an essential step to understanding cellular contributions to development, aging, injury, and disease. Since proteome and transcrip-tome level abundances modestly correlate, complementary profiling of both is needed. We report a method called simultaneous protein and RNA -omics (SPARO) to capture the cell type-specific transcriptome and proteome simultaneously in vitro and in vivo. SPARO leverages TurboID to biotinylate RNA-interacting cytosolic proteins, enabling enrichment of proteins for proteomics and protein-associated RNA for transcriptomics. We validate SPARO first using well-controlled in vitro systems to verify that the proteomes and transcriptomes obtained reflect the global proteo-mes and transcriptomes. The effect of neuroinflammatory activation by lipopolysaccharide is al-so faithfully captured. We apply SPARO to obtain native-state proteomes and transcriptomes from astrocytes and neurons, thereby validating the approach in vivo. We interrogate mRNA-protein concordance and discordance, providing insights into molecular processes that exhibit uniform or cell type-specific patterns.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE287770 | GEO | 2026/02/16

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2026-02-16 | PXD073954 | Pride
2026-02-16 | PXD059818 | Pride
2020-04-20 | GSE120379 | GEO
2023-03-11 | PXD036744 | Pride
2024-06-16 | GSE229334 | GEO
2024-01-09 | GSE252575 | GEO
2019-04-18 | GSE128812 | GEO
2013-05-28 | E-MTAB-1153 | biostudies-arrayexpress
2012-10-26 | E-MTAB-1154 | biostudies-arrayexpress
2025-05-01 | GSE262690 | GEO