Transcriptomics

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Single-cell multiomic characterization of erythroid cells (cultured human BEL-A cell line) with a panel of 118 genes and 7 surface markers on the BD Rhapsody platform


ABSTRACT: We used the BD Rhapsody multi-omics platform to perform a targeted quantification of 118 erythroid mRNA species and seven erythroid surface proteins in developing erythroid cells derived from the human erythroid cell line BEL-A. The goal of the project was to detect heterogeneity among developing human red blood cells independent of their state of maturity and relate this to their expression of fetal haemoglobin-specific globin chains. A gene-targeted approach was selected to increase the power to evaluate ranscripts of interest and to exclude alpha and beta globin mRNA, which tend to swamp experimental data in more mature precursors and reticulocytes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE287830 | GEO | 2025/06/18

REPOSITORIES: GEO

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