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Unveiling the response of efflux pump genes to delamanid in Mycobacterium tuberculosis H37Rv: transcriptomic analysis.

ABSTRACT: Efflux pump overexpression is associated with drug resistance in Mycobacterium tuberculosis, but its role in delamanid resistance is not well understood. This study sought to identify efflux pump genes affected by delamanid exposure using transcriptomic analysis. Cultures of M. tuberculosis H37Rv were exposed to delamanid at a sub-inhibitory concentration of ½ MIC for 24 hours, and transcriptomic sequencing was conducted on both treated and untreated samples. Differentially expressed genes (DEGs) were annotated using the UniProt database. The analysis revealed significant upregulation of 23 efflux pump genes under delamanid stress, with 73.9% belonging to the ABC family, 17.39% to the RND family, and 8.69% to the MFS superfamily. These results highlight efflux pump genes that are responsive to sub-inhibitory levels of delamanid.

ORGANISM(S): Mycobacterium tuberculosis H37Rv

PROVIDER: GSE287913 | GEO | 2026/04/30

REPOSITORIES: GEO

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