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TRNA m1A modification in macrophages promotes translation of interferon pathway genes to enhance CD8+ T cell-mediated anti-tumor immunity [CAR-iMACs RNA-seq]

ABSTRACT: The involvement of tRNA m1A modification in the anti-tumoral tumor-associated macrophages (TAMs) has been validated in our study that macrophage-specific knockout of m1A “writer” enzymes TRMT61A leads to increased infiltration of TAMs, impaired CD8+ T cell function, and enhanced tumor growth. Since we observed that tumor-derived components suppresses tRNA m1A modification in macrophages, we sought to identify which component in the TME induces the down-regulation of tRNA m1A modification. Here, we performed bulk RNA-seq using TRMT61AOE-CAR-iMACs and CAR-iMACs to ensure the effect of human Trmt61a overexpression on CAR-iMACs. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that signal transduction is the most up-regulated pathway in CAR-iMACs after human Trmt61a overexpression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE288142 | GEO | 2026/03/04

REPOSITORIES: GEO

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