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Changes in the Bone Marrow Microenvironment Impact Anabolic Actions of PTH

ABSTRACT: Irradiation induced bone marrow ablation ultimately enhanced PTH anabolic effects in bone. B6 mice at 10 days of age were sub-lethally irradiated and treated with PTH 24h later for 5 days. 24h post last-injection, bone marrow was flushed with Trizol and RNA isolated and purified. Microarray analyses was performed to determine differential differences in PTH effects in non-irradiated vs. irradiated bone marrow.

ORGANISM(S): Mus musculus

PROVIDER: GSE28819 | GEO | 2011/09/30

SECONDARY ACCESSION(S): PRJNA138773

REPOSITORIES: GEO

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Changes in the Bone Marrow Microenvironment Impact Anabolic Actions of PTH

Project description:Irradiation induced bone marrow ablation ultimately enhanced PTH anabolic effects in bone. B6 mice at 10 days of age were sub-lethally irradiated and treated with PTH 24h later for 5 days. 24h post last-injection, bone marrow was flushed with Trizol and RNA isolated and purified. Microarray analyses was performed to determine differential differences in PTH effects in non-irradiated vs. irradiated bone marrow. Triplicates of 4 groups (total of 12 samples) which include: Nonirradiated Vehicle, Nonirradiated PTH, Irradiated Vehicle and Irradiated PTH

2011-09-29 | E-GEOD-28819 | biostudies-arrayexpress

Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

Project description:To investigation the role of PTH and Kindlin-2 in bone development, we performed single-cell RNA-sequencing. From Con-veh, Con-PTH, cKO-veh, cKO-PTH, we profiled more than 20k single cells, including multi-potent mesenchymal stromal cells (MSC), osteoprogenitors, osteoblasts, chondrocytes, fibroblasts, endothelial cells, smooth muscle cells, skeletal muscle cells, pericytes, and schwann cells. We found proportion of part of these cells were significant altered by PTH or Kindlin-2 loss, especially for MSC, osteoblast, chondrocyte, and fibroblast. Transcriptomic analysis revealed gene expression was dramatically regulated by PTH or Kindlin-2 loss.

2020-10-01 | GSE150291 | GEO

Mesenchymal Stem Cells (MSCs): Control vs. PTH-stimulated

Project description:Transcriptional profiling of human MSCs comparing control MSCs with parathyroid hormone (PTH)-stimulated MSCs. PTH-stimulated MSCs were treated with 0.1 nM recombinant human PTH (N-terminal fragment, amino acids 1-34) for 48 hours. Human MSCs were isolated from a bone marrow sample obtained from a healthy adult volunteer.

2014-02-01 | GSE43148 | GEO

Mesenchymal Stem Cells (MSCs): Control vs. PTH-stimulated

2014-02-01 | E-GEOD-43148 | biostudies-arrayexpress

Expression data from placenta harvested from WT and Pth-null fetuses treated 90 minutes prior with saline or PTH (1-84)

Project description:Parathyroid hormone (PTH) plays an essential role in regulating calcium and bone homeostasis in the adult, but whether PTH is required at all for regulating fetal-placental mineral homeostasis is uncertain. To address this we treated Pth-null mice in utero with 1 nmol PTH (1-84) or saline and examined placental calcium transfer 90 minutes later. It was found that placental calcium transfer increased in Pth-null fetuses treated with PTH as compared to Pth-null fetuses treated with saline. Subsequently, to determine the effect of PTH treatment on placental gene expression, in a separate experiment, 90 minutes after the fetal injections the placentas were removed for subsequent RNA extraction and microarray analysis.

2010-03-01 | GSE16983 | GEO

Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

Project description:Kindlin-2 modulates PTH1R to regulate bone mass and PTH actions in bone

| PRJNA631725 | ENA

An irradiation-altered bone marrow microenvironment impacts anabolic actions of PTH.

Project description: Not available

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Anabolic actions of PTH in murine models: two decades of insights.

Project description: Not available

| S-EPMC8596798 | biostudies-literature

RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice

Project description:C57Bl/6 Ly5.1 male mice lethally irradiated have been transplanted at 8 weeks of age with bone marrow from Hbb(th3/+)/Tfr2(+/+) or Hbb(th3/+)/Tfr2(-/-) mice (Bone marrow in a mixed genetic background C57BL6/129Sv). After transplantation, the mice have been maintained in a standard diet for 22 weeks and euthanised for organs extraction and analysis.

2020-03-04 | E-MTAB-6606 | biostudies-arrayexpress

Screening RIPK3 dependent signaling pathway and genes expression in intestinal GVHD mice models

Project description:Lethally irradiated recipient C57BL/6 WT, RIPK3-/- and Villin-cre RIPK3fl/fl mice received Balb/c bone marrow and CD4+ T cells. After 17 days, total RNA was extracted from small intestine and deep sequenced to compare the gene expression profiles among WT, RIPK3-/- and Villin-cre RIPK3fl/fl.

2021-01-28 | GSE165606 | GEO

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