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Transcriptome analysies reveals the role of OsCBM1 in rice defense against Xanthomonas oryzae pv.oryzae

ABSTRACT: Carbohydrate-binding malectin/malectin-like domain-containing proteins (CBMs) represent a newly discovered subfamily of lectins that are involved in a range of bio-logical processes across the animal, bacterial, and plant kingdoms. The OsCBM1 gene in rice enhances reactive oxygen species (ROS) burst, in response contributing to drought-stress tolerance. Nonetheless, the functions of OsCBM1 response to biotic stress remain poorly understood. In this study, we found that OsCBM1 was induced by the infection of Xoo and overexpression of OsCBM1 enhances rice resistance to the bacterial blight, while suppression of its expression shows the opposite trend. OsCBM1 may influence resistance to bacterial blight by regulating ROS burst and the SA sig-naling pathway through RNA-seq analysis. Overexpression of OsCBM1 increased SA content and enhanced activities of SOD, POD, and CAT enzymes, whereas knockdown of OsCBM1 exhibited the opposite trend. The expression of genes related to the SA and enzyme activity pathways was validated through qPCR. These results further eluci-date the function of OsCBM1 in biotic stress resistance, providing references for dis-ease-resistant rice breeding.

ORGANISM(S): Oryza sativa

PROVIDER: GSE288740 | GEO | 2025/03/05

REPOSITORIES: GEO

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Project description:As sessile organism, plants evolved a highly complicated signaling system to cope with unfavorable and fluctuating environmental conditions. Rapid and transient Reactive Oxygen Species (ROS) burst is a common response to both biotic and abiotic stresses. Plants exposed with O3 could trigger extracellular similar ROS production through cell wall peroxidases and NPADPH oxidases, resulting in changes in the gene expression and cell death. Whereas ROS induced cell death is not simply due to its toxicity, rather due to interplay with several other signaling pathways, such as salicylic acid (SA), jasmonic acid (JA) and ethylene signaling pathways. Furthermore, the three hormones have both synergistic and antagonistic interactions, where the suppression of JA signaling by SA is the mostly studied. In addition, ethylene promotes cell death while JA has a protective role upon O3 exposure. The role of SA is more complicated; depending on the genetic background it can have either cell death promoting or protecting roles. Hence, a clean system to deliver apoplastic ROS is required to study the role of ROS apart from con-current activation of other signaling pathways. Arabidopsis thaliana offer a convenient system to study apoplastic ROS signaling due to the availability of hormone signaling or biosynthesis mutants including the JA receptor mutant coi1-16 (CORONATINE INSENSITIVE1), the essential ethylene signaling mutant ein2 (ETHYLENE INSENSITIVE2), the SA biosynthesis mutant sid2 (SALICYLIC ACID INDUCTION DEFICIENT2 also known as ISOCHORISMATE SYNTHASE1), and essential regulators in SA/JA/ethylene-induced defense response triple mutant tga2 tga5 tga6 (Clade II TGA transcription factors). Here we used a combination of transcriptome analysis, cell death assays and mutant analysis to systematically quantified the contribution of hormone signaling in relation to apoplastic ROS signaling, identified transcription factors (TFs) involved in ROS regulation and dissected the components involved in defense hormones associated cell death. Transcriptome profiling of ozone response using two arabidopsis triple mutants coi1-16 ein2 sid2 and tga2 tga5 tga6 related to Jasmonic acid, salicylic acid and ethylene signaling to identify hormone-independant apoplastic ROS signaling

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Transcriptome analysies reveals the role of OsCBM1 in rice defense against Xanthomonas oryzae pv.oryzae

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