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Single-Cell RNA Sequencing of Bone Marrow ILC2p and CHILPs, and Lung and Mesenteric Lymph Node ILC2s

ABSTRACT: To explore potential tissue origins of iILC2 during helminth infection, we performed single-cell RNA sequencing (scRNA-seq) of ILC2 and ILC progenitors using the 10x Genomics platform. Intestine draining mesenteric lymph nodes (Mes LN), lung, and bone marrow (BM) were collected 5 days post infection (dpi) with Nippostrongylus brasiliensis.

ORGANISM(S): Mus musculus

PROVIDER: GSE288743 | GEO | 2025/06/12

REPOSITORIES: GEO

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Human resistin alters lung mRNA expression from helminth-infected lungs.

Project description:Goal: To examine the effects of human resistin during helminth infection. Methodology: To examine the function of human resistin (hResistin), we utilized transgenic mice expressing the human resistin gene along with its entire regulatory region (hRetnTg+). Following infection with the helminth Nippostrongylus brasiliensis, whole lung RNA was sequenced in hRetnTg+ mice, control hRetnTg- and naïve mice. Conclusion: In hRetnTg+ mice, many genes involved in inflammation and the immune system, specifically toll-like receptor signaling and chemokines, are significantly upregulated, suggesting that human resistin promotes TLR signaling and inflammation during helminth infection. Examination of whole lung mRNA from Nippostrongylus brasiliensis-infected lungs at day 7 in mice expressing human resistin

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