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Nascent-seq of HeLa WT and SRSF5 KO cells

ABSTRACT: We used HeLa cells where SRSF5 was knocked out by CRISPR/Cas9 to perform Nascent-seq. WT cells served as control. One hour before harvesting, cells were incubated with 4-sU for exactly 1 hour to label newly transcribed RNAs. 4-sU-labelled RNAs were biotinylated and purified over streptavidin beads and transformed into an RNA-seq library. We compared differential expression and intron retention between WTand SRSF5 KO samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE288960 | GEO | 2026/01/29

REPOSITORIES: GEO

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