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The mechanotransducer Piezo1 coordinates metabolism and inflammation to promote skin regeneration.

ABSTRACT: The skin has remarkable ability to grow under constant stretch. We developed a mouse model to imitate human tissue expansion to study the cellular and molecular mechanisms involved in tension-induced skin growth. We examined specific pathway changes within the entire skin at both the spatial and single-cell level. Our analysis has revealed the fractions of basal and suprabasal cells increased after expansion. We observed elevated infiltration of immune cells in the skin, suggesting a tension-induced skin inflammatory response.

ORGANISM(S): Mus musculus

PROVIDER: GSE289263 | GEO | 2025/05/01

REPOSITORIES: GEO

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Single Cell RNA-sequencing of murine back skin interfollicular epidermis basal cells

Project description:The ability of the skin to grow in response to stretching has, for decades, been exploited in reconstructive surgery. The response of epidermal cells to stretching has been studied in vitro. However, it remains unclear how mechanical forces affect epidermal cell behaviour in vivo. Here, we develop a mouse model in which the consequences of stretching on skin epidermis can be studied. Using a multidisciplinary approach that combines clonal analysis with quantitative modelling and single-cell RNA-seq, we show that stretching induces skin expansion by a transient bias in the renewal activity of epidermal stem cells, while a second subpopulation of basal progenitors remains committed to differentiation. Transcriptional and chromatin profiling identifies how cell states and gene regulatory networks are modulated by stretching. Using pharmacological inhibitors and mouse mutants, we define the mechanisms that control stretch-mediated tissue expansion.

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