Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An ancient and essential miRNA family controls cellular interaction pathways in C. elegans [miRNA-Seq]

ABSTRACT: The transition from unicellular to multicellular life required the acquisition of coordinated and regulated cellular behaviors, including adhesion and migration. In metazoans, this relies on cell adhesion proteins, signaling systems, and an elaborate extracellular matrix (ECM) that contributes to cell adhesion and to the milieu in which signaling interactions occur. Innovations in these pathways that enabled complex multicellularity occurred at the level of new genes, new functions for existing genes, but also at the regulatory level. Gene regulation by microRNAs expanded with the evolution of multicellularity, but the functions of individual microRNAs in this context are largely unexplored. A single microRNA, miR-100, arose in the last common eumetazoan ancestor and is widely conserved across animals. Here, we reveal the molecular function of the homolog of miR-100 in C. elegans, the miR-51 family. The miR-51 family acts in a dose dependent manner to control morphogenesis by regulating several genes involved in cell signaling, adhesion and migration, including modifiers of the ECM, specifically heparan sulfate sulfotransferases (HSTs). Specific HSTs and signaling pathway components are also predicted conserved targets of miR-100 across vertebrates. Our work suggests that this miRNA provided an innovation in the regulation of cellular interactions early in metazoan evolution, as animals evolved to form more complex bodies.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE289510 | GEO | 2025/07/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

An ancient and essential miRNA family controls cellular interaction pathways in C. elegans

Project description:The transition from unicellular to multicellular life required the acquisition of coordinated and regulated cellular behaviors, including adhesion and migration. In metazoans, this relies on cell adhesion proteins, signaling systems, and an elaborate extracellular matrix (ECM) that contributes to cell adhesion and to the milieu in which signaling interactions occur. Innovations in these pathways that enabled complex multicellularity occurred at the level of new genes, new functions for existing genes, but also at the regulatory level. Gene regulation by microRNAs expanded with the evolution of multicellularity, but the functions of individual microRNAs in this context are largely unexplored. A single microRNA, miR-100, arose in the last common eumetazoan ancestor and is widely conserved across animals. Here, we reveal the molecular function of the homolog of miR-100 in C. elegans, the miR-51 family. The miR-51 family acts in a dose dependent manner to control morphogenesis by regulating several genes involved in cell signaling, adhesion and migration, including modifiers of the ECM, specifically heparan sulfate sulfotransferases (HSTs). Specific HSTs and signaling pathway components are also predicted conserved targets of miR-100 across vertebrates. Our work suggests that this miRNA provided an innovation in the regulation of cellular interactions early in metazoan evolution, as animals evolved to form more complex bodies.

2025-07-10 | GSE289436 | GEO

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi [Phanerochaete chrysosporium]

Project description:The evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple multicellular aggregates of cells, it has evolved only in a handful of lineages, including the animals, embryophytes, red and brown algae and fungi. Despite being a key step towards the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall (FCW) remodeling, targeted protein degradation, signal transduction, adhesion and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, with from which many convergently expandedwere identified in multicellular plants and/or animals too, assuming convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. This study provides a novel entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms.

2019-03-16 | GSE125199 | GEO

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi [Schizophyllum commune]

2019-03-16 | GSE125198 | GEO

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi [Rickenella mellea]

2019-03-16 | GSE125195 | GEO

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi [Lentinus tigrinus]

2019-03-16 | GSE125190 | GEO

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi [Coprinopsis cinerea]

2019-03-16 | GSE125184 | GEO

Depletion of beta1 integrins in triple negative breast cancer cells causes extensive alterations in gene expression profiles

Project description:Interactions with the extracellular matrix (ECM) through integrin adhesion receptors provide cancer cells with physical and chemical cues that act in concert with growth factors to support survival and proliferation. Preclinical studies testing beta1 integrin antagonists in (breast) cancer models have shown inhibition of tumor growth and sensitization to radio- or chemotherapy and these strategies are currently evaluated in clinical trials. Here, we show that disruption of beta1 integrin-mediated ECM adhesion attenuates breast tumor growth but dissemination to the lungs from such small tumors can be markedly enhanced. beta1 integrin downregulation induces compensatory upregulation of beta3 integrins, but increased beta3 expression does not lead to enhanced lung metastasis. Instead, beta1 integrin downregulation in human and mouse triple negative, E-cadherin positive breast cancer cells elicits a switch from collective invasion to individual cell migration in 3D ECM. This involves alterations in the TGFbeta-BMP signaling network shifting the balance between miR-200 and ZEB, which causes a block in E-cadherin transcription. The switch is fully reversible: restored beta1 expression reinstates E-cadherin expression and cell cohesion. Moreover, restoring the network at the level of TGFbetaR, ZEB/miR-200 balance, or E-cadherin, restores cohesion and prevents the induction of lung metastasis without affecting tumor growth. These findings reveal that integrin-mediated ECM-attachments regulate a signaling network in control of epithelial characteristics that suppress metastatic spread. This raises concerns with respect to the use of beta1 integrins as cancer drug targets

2014-02-11 | E-MTAB-2185 | biostudies-arrayexpress

Evolution of human-specific microRNA miR-941 [miRNA transfection]

Project description:miRNA-mediated gene expression silencing has previously been shown to be important for a variety of physiological and pathological processes. Here, we have explored the role of one bona fide human-specific miRNA (miR-941) in evolution of the human-specific expression and function. Using combination of high-throughput sequencing (GSE26545), miRNA transfection and large-scale PCR of various human populations, we have shown that emergence and rapid expansion of miR-941 might take place on the human evolutionary linage between six and one million years ago. Functionally, miR-941 could be associated with hedgehog and insulin signaling pathways, and thus might potentially play a role in evolution of human longevity. Human-specific effects of miR-941 regulation are detectable in human brain and affect genes involved in neurotransmitter signaling. Furthermore, emergence of miR-941 on the human evolutionary linage was accompanied by the accelerated loss of its binding sites. Taken together, these results strongly implicate the contribution of miR-941 in evolution of the human-specific phenotype.

2012-12-20 | GSE35620 | GEO

miRNA Lisbon

Project description:Proteomic analysis of differentially expressed proteins in MDA-MB-231 and MCF-10A cell lines when miR-200c and miR-203 were transiently expressed or inhibited, respectively.

2022-02-28 | PXD007210 | Pride

Project description:Experimental evolution of multicellularity

| PRJNA693121 | ENA