High throughput identification of genetic regulators of microglial inflammatory processes in Alzheimer's disease.

ABSTRACT: We sought to assess at scale how AD GWAS hits influence human microglial inflammatory responses. We conducted CRISPR inhibition screens of 119 AD GWAS hits in parallel along with 52 control genes in human iPSC-derived microglia, with reactive oxygen species produced in response to the viral mimic poly(I:C) as a readout. Top hits that either decreased or increased ROS in response to poly(I:C) when knocked down were then interrogated via perturb-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE289721 | GEO | 2025/12/31

REPOSITORIES: GEO