Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression microarray for mouse ES cells vs. mouse ES cells treated with 48 hr N2B27

ABSTRACT: Core circuits of transcription factors stabilize stem and progenitor cells by suppressing genes required for differentiation. We do not know how such core circuits are reorganized during cell fate transitions to allow differentiation and lineage choice to proceed. Here, we asked how the pluripotency circuit, a core transcriptional circuit that maintains mouse embryonic stem (ES) cells in a pluripotent state, is dismantled as ES cells differentiate and choose between the neural ectodermal and mesendodermal progenitor cell fates. When ES cells are recultured from pluripotency maintaining conditions to the basal media N2B27, the expression of the pluripotency circuit genes begins to change. At 48 hours post N2B27 addition, the ES cells are competent to respond to differentiation signals. Here, our microarray analysis compares the gene expression profile of ES cells vs. the gene expression profile of cells that have been treated with N2B27 for 48 hours, reaching the competent state.

ORGANISM(S): Mus musculus

PROVIDER: GSE29005 | GEO | 2011/05/03

SECONDARY ACCESSION(S): PRJNA140635

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression microarray for mouse ES cells vs. mouse ES cells treated with 48 hr N2B27

Project description:Core circuits of transcription factors stabilize stem and progenitor cells by suppressing genes required for differentiation. We do not know how such core circuits are reorganized during cell fate transitions to allow differentiation and lineage choice to proceed. Here, we asked how the pluripotency circuit, a core transcriptional circuit that maintains mouse embryonic stem (ES) cells in a pluripotent state, is dismantled as ES cells differentiate and choose between the neural ectodermal and mesendodermal progenitor cell fates. When ES cells are recultured from pluripotency maintaining conditions to the basal media N2B27, the expression of the pluripotency circuit genes begins to change. At 48 hours post N2B27 addition, the ES cells are competent to respond to differentiation signals. Here, our microarray analysis compares the gene expression profile of ES cells vs. the gene expression profile of cells that have been treated with N2B27 for 48 hours, reaching the competent state. 2 x mouse ES cells in pluripotency maintaining conditions. 3 x mouse ES cells after 48 hr of N2B27 culture

2011-05-03 | E-GEOD-29005 | biostudies-arrayexpress

ES, EC, NS, TS, Placenta and Neural induction (N2B27) comaprison

Project description:Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction. Keywords: cell type comparison design,development or differentiation design,time series design

2006-01-23 | GSE4077 | GEO

ES, EC, NS, TS, Placenta and Neural induction (N2B27) comaprison

Project description:Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction. Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction.

2010-06-11 | E-GEOD-4077 | biostudies-arrayexpress

Neural induction (N2B27 monolayer cluture)

Project description:Neural induction of ES by N2B27 monolayer culture Keywords: development or differentiation design,time series design

2006-01-23 | GSE4076 | GEO

Transcription profiling of mouse ES, EC, NS, TS, placenta and neural induction (N2B27) comaprison

Project description:Comparison among ES, EC, TS, NS, differentiated neural cells derived from NS and placenta in addition to ES-N2B27 neural induction.

2005-01-01 | E-MNIA-65 | biostudies-arrayexpress

Transcription profiling of mouse ES cell neural induction (N2B27 monolayer cluture)

Project description:Neural induction of ES by N2B27 monolayer culture

2005-01-01 | E-MNIA-64 | biostudies-arrayexpress

Neural induction (N2B27 monolayer cluture)

Project description:Neural induction of ES by N2B27 monolayer culture Neural induction of ES by N2B27 monolayer culture

2006-01-22 | E-GEOD-4076 | biostudies-arrayexpress

Expression profile of MEFs, iPS, and ES in different culture condition

Project description:MEFs can be reprograming to ES by defined reprogramming factors, the derived ES-like cells were termed iPSc. Completely reprogrammed iPSc have identifed gene expression profle with ES. Culture condition may influent the pluripotency of iPSCs.iPS/ES cultured in differient ES medium may have different gene expression profile. We preform an experiment to analyze the gene expression difference among MEFs cultured in FBS and iCD1,iPSc cultured in KSR medium and N2B27 supplementary with 2i medium , and ES cultured in N2B27 supplementary with 2i medium.

2010-10-27 | E-GEOD-24861 | biostudies-arrayexpress

TG-interacting factor1 (Tgif1) maintains the identity of mouse ES cells by counterbalancing the expression of ES cell core factors

Project description:TG-interacting factor1 (Tgif1) is well-known as a transcriptional repressor in transforming growth factor beta (TGFβ) signaling pathway. Target mapping of ES cell core factors in mouse embryonic stem (ES) cells revealed that Tgif1 is occupied by Oct4 and Nanog. Moreover, recent interactome study of mouse gene regulatory regions showed a preferential regulation of Tgif1 by mouse ES cell specific enhancers. However, the detailed role and mode of actions of Tgif1 in stem cell maintenance and development remains elusive. We show that Tgif1 is indispensable for self-renewal and pluripotency of mouse embryonic stem (ES) cells. Aberrant expression of Tgif1 promotes differentiation of ES cells even in the presence of LIF in part by deregulation of pluripotency factors. Intriguingly, we find that Tgif1 level is a critical factor to determine specific lineage commitment in a dosage-dependent manner. We further show that Tgif1 interacts with ES cell core factors and co-localizes at their binding sites, which eventually restricts expression of ES cell core factors including Oct4, Sox2, and Nanog. Taken together, we provide new insights into the roles of Tgif1 in maintenance as well as differentiation of ES cells.

2015-09-26 | GSE55404 | GEO

GlcNAcstatin treatment of ES cells

Project description:Effect of GlcNAcstatin on mouse ES cells and during the first 24h of N2B27 neural differentiation

2014-03-01 | E-MEXP-3593 | biostudies-arrayexpress