In vivo CRISPR screens of Toxoplasma gondii targeting proteins of the apical complex and the pellicle.
Ontology highlight
ABSTRACT:
ORGANISM(S): Toxoplasma gondii
PROVIDER: GSE290078 | GEO | 2026/03/06
REPOSITORIES: GEO
Similar Datasets
Project description:In vivo CRISPR screens of Toxoplasma gondii targeting proteins of the apical complex and the pellicle.
| PRJNA1226160 | ENA
Project description:Toxoplasma gondii is an obligate intracellular parasite. The ability to invade cells and proliferate successfully is given by the machinery found in the pellicle of the parasite, the pellicle is a complex of three membranes. Despite the importance of the pellicle in the biology of Toxoplasma, its protein composition had not been reported. The proteins of the isolated pellicle fraction were digested with trypsin and chymotrypsin, for their identification and quantification in the case of those digested with trypsin and only identification in the case of those digested with chymotrypsin. Samples were resolved, and peptides were separated using a UHPLC ACQUITY M-Class. Spectral data were acquired in an MS with electrospray ionization and ion mobility separation Synapt G2-Si operated with data-independent acquisition and ion mobility spectrometry using the high-definition multiplexed MS/MS mode. We used the Hi3 method to quantify absolutely every protein detected. In the isolate of pellicle digested with trypsin a total of 548 proteins were identified and absolutely quantified using the internal standard and the intensity of the most abundant peptides per protein with Progenesis QI software and the database UP000002494. Among identified proteins, 11 corresponded to IMC, 11 were related to SAG (SRS), 15 to plasma membrane and glideosome and 13 to cytoskeleton (CK). In the chymotrypsin-digested pellicle sample a total of 137 proteins were identified and 22 of them were proteins that were not identified in the trypsin-digested sample. Due to the characteristics of the SRS proteins, they have a role in generating the host's immune response or are involved in adhesion, motility and invasion. The 11 proteins found with the analysis of E. mass. Study of these SRS proteins to determine their role in the dynamic events of the parasite, such as adhesion and motility as well as in the generation of the immune response, is important in the search for therapies against toxoplasmosis
2021-02-16 | PXD022398 | Pride
Project description:We performed in vivo CRISPR screens of Toxoplasma targeting hyperLOPIT-unassigened genes during mouse infection.
2024-06-30 | GSE253885 | GEO
Project description:We performed in vivo CRISPR screens of Toxoplasma targeting genes with hyperLOPIT-unassigened subcellular localisation during mouse infection.
2024-06-30 | GSE253884 | GEO
Project description:One of the most distinct features of Pseudoalteromonas sp. SCSIO 11900 is its ability to form a very robust pellicle than most Pseudoalteromonas strains. Thus we want to identify the genes essential for the pellicle formation of SCSIO 11900. We compared transcriptom profiles of planktonic cells, initial pellicle and mature pellicle of coral Pseudoalteromonas sp. SCSIO 11900 and revealed that some unique genes from horizontal gene transfer is involved in the pellicle formation of SCSIO 11900.
2017-04-04 | GSE97322 | GEO
Project description:Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. In recent years, biofilm development of S. oneidensis has been extensively studied because it is essential to reduce solid metals. As a special form of biofilm, however, pellicles are largely overlooked. The goal of this work was to understand requirements of S. oneidensis pellicle formation and the molecular basis of pellicle formation. We demonstrated that successful pellicle formation and survival was likely to require the threshold level of cell density and higher concentration of oxygen. Proteinase K and EDTA were potent pellicle disrupter. DNA microarray experiments were used to study the gene expression profile of young air–liquid interface pellicle relative to planktonic cells, which indicated that the air–liquid interface pellicle was more metabolically active than the planktonic cells. Most notably, consistently up-regulation of iron or heme uptake and transportation proteins was observed in the S. oneidensis MR-1 pellicle. However, neither the hmuT nor hugA heme transport mutant was defective in pellicle formation. An examination of the influence of several metal cations on the anti-pellicle activity of EDTA showed that Ca (II), Mn(II), Cu(II), and Zn(II) fully protected S. oneidensis MR-1 pellicle against EDTA treatment while additional of iron enabled the initiation of pellicle formation but maturation was significantly impaired. Collectively, iron was less important than other metals with respect to pellicle formation in S. oneidensis.
2010-09-01 | GSE14331 | GEO
Project description:the proteins of Toxoplasma gondii interacting with mouse NLRP3
2021-12-21 | MSV000088602 | MassIVE
Project description:CRISPR-Cas9 screens to identify regulators of differentiation in Toxoplasma gondii
| PRJNA546577 | ENA
Project description:Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse respiratory capabilities. In recent years, biofilm development of S. oneidensis has been extensively studied because it is essential to reduce solid metals. As a special form of biofilm, however, pellicles are largely overlooked. The goal of this work was to understand requirements of S. oneidensis pellicle formation and the molecular basis of pellicle formation. We demonstrated that successful pellicle formation and survival was likely to require the threshold level of cell density and higher concentration of oxygen. Proteinase K and EDTA were potent pellicle disrupter. DNA microarray experiments were used to study the gene expression profile of young air–liquid interface pellicle relative to planktonic cells, which indicated that the air–liquid interface pellicle was more metabolically active than the planktonic cells. Most notably, consistently up-regulation of iron or heme uptake and transportation proteins was observed in the S. oneidensis MR-1 pellicle. However, neither the hmuT nor hugA heme transport mutant was defective in pellicle formation. An examination of the influence of several metal cations on the anti-pellicle activity of EDTA showed that Ca (II), Mn(II), Cu(II), and Zn(II) fully protected S. oneidensis MR-1 pellicle against EDTA treatment while additional of iron enabled the initiation of pellicle formation but maturation was significantly impaired. Collectively, iron was less important than other metals with respect to pellicle formation in S. oneidensis. A fresh colony grown overnight on a LB plate was used to inoculate 50 ml LB and incubated in a shaker (200 rpm) to an OD600 of 0.8 at the room temperature. This culture was then diluted 500-fold with fresh LB, resulting in the starting cultures. Aliquots of 30ml starting cultures were transferred to 50-ml Pyrex beakers and allowed to develop pellicles at the room temperature. When a complete but thin (young pellicle) at the interface were formed (about 30h hours), planktonic culture and pellicle were separated and applied to centrifugation at 8000 rpm for 3 min at room temperature. 3 parallel starting cultures were used and 3 samlpes of pellicle cells or planktonic cells were collected at 30h. RNA from the pellicle cells was fluorescently labeled with Cy3, and that from the planktonic was labeled with Cy5.
2010-09-01 | E-GEOD-14331 | biostudies-arrayexpress