CDNA array expression profile of wild-type and fl-mutant upland cotton ovules
Ontology highlight
ABSTRACT:
ORGANISM(S): Gossypium hirsutum
PROVIDER: GSE2901 | GEO | 2006/01/01
SECONDARY ACCESSION(S): PRJNA92599
REPOSITORIES: GEO
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Project description:We constructed cDNA library from 0-10 day post anthesis cotton fibers when fiber cells begin to initiate and elongate throughout this peroid. We randomly sequenced over 95,000 ESTs from this library, and with request of upland cotton ESTs from other laboratories, we acquired a gene pool of more than 30,000 UniESTs. The cotton UniESTs were then PCR-amplified and printed onto microarray. This array is comprised of about 29,000 high-quality cotton cDNAs (each sequence length>400bp, average length > 600bp) and external controls. Keywords: repeat samples for transcriptome analysis
2008-04-01 | GSE8443 | GEO
Project description:Transcriptomes fiber and ovules were compared by applying serial analysis of gene expression (SAGE). Keywords: Tissue Comparison We constructed three SAGE libraries and sequenced 57321, 64188, and 69104 tags from fiber, Xu-142 ovule (ovule) and fl mutant ovules (fl) respectively of Upland Cotton, Gossypium hirsutum L. cv. Xu-142.
2007-02-17 | E-GEOD-6103 | biostudies-arrayexpress
Project description:A cDNA library from 0-10 day post anthesis cotton ovules was established to study genes expressed in cotton ovule during initiation and quickly elongation period. We randomly sequenced over 100,000 ESTs from this library and acquired a gene pool of more than 28,000 UniESTs. The cotton UniESTs were then PCR-amplified and printed onto microarray. This array is comprised of about 28000 high-quality cotton cDNAs (with average length>750bp) and external controls. To study the different growth potential of cotton fibers in a one-year cycle, we then hybridized the array with RNA samples derived from +7 DPA wild-type upland cotton fibers in four different seasons, respectively.
2010-06-01 | GSE16802 | GEO
Project description:Comparison of gene expression profiles of wild-type and fl-mutant upland cotton ovules Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+5-Cy5 wt+3-Cy3 Set 1 (dye swap): wt+5-Cy3 wt+3-Cy5 Set 2: wt+5-Cy5 wt+3-Cy3 Set 2 (dye swap): wt+5-Cy3 wt+3-Cy5 Set 3: wt+5-Cy5 wt+3-Cy3 Set 3 (dye swap): wt+5-Cy3 wt+3-Cy5 GSM63341, GSM63342, GSM63343, GSM63344, GSM63345, GSM63346 Data for 3 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+3-Cy5 vs wt+3-Cy3 Set 2: wt+3-Cy3 vs wt+3-Cy5 Set 3: wt+3-Cy5 vs wt+3-Cy3 GSM63365, GSM63366, GSM63367 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+3-Cy5 wt-3-Cy3 Set 1 (dye swap): wt+3-Cy3 wt-3-Cy5 Set 2: wt+3-Cy5 wt-3-Cy3 Set 2 (dye swap): wt+3-Cy3 wt-3-Cy5 Set 3: wt+3-Cy5 wt-3-Cy3 Set 3 (dye swap): wt+3-Cy3 wt-3-Cy5 GSM63368, GSM63369, GSM63370, GSM63371, GSM63372, GSM63373 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: 0wt-Cy5 wt+3-Cy3 Set 1 (dye swap): 0wt-Cy3 wt+3-Cy5 Set 2: 0wt-Cy5 wt+3-Cy3 Set 2 (dye swap): 0wt-Cy3 wt+3-Cy5 Set 3: 0wt-Cy5 wt+3-Cy3 Set 3 (dye swap): 0wt-Cy3 wt+3-Cy5 GSM63374, GSM63375, GSM63376, GSM63377, GSM63378, GSM63379 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+3-Cy5 fl+3-Cy3 Set 1 (dye swap): wt+3-Cy3 fl+3-Cy5 Set 2: wt+3-Cy5 fl+3-Cy3 Set 2 (dye swap): wt+3-Cy3 fl+3-Cy5 Set 3: wt+3-Cy5 fl+3-Cy3 Set 3 (dye swap): wt+3-Cy3 fl+3-Cy5 GSM63391, GSM63392, GSM63393, GSM63394, GSM63395, GSM63396 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+10-Cy5 fl+10-Cy3 Set 1 (dye swap): wt+10-Cy3 fl+10-Cy5 Set 2: wt+10-Cy5 fl+10-Cy3 Set 2 (dye swap): wt+10-Cy3 fl+10-Cy5 Set 3: wt+10-Cy5 fl+10-Cy3 Set 3 (dye swap): wt+10-Cy3 fl+10-Cy5 GSM63397, GSM63398, GSM63399, GSM63400, GSM63401, GSM63402 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+10-Cy5 wt+3-Cy3 Set 1 (dye swap): wt+10-Cy3 wt+3-Cy5 Set 2: wt+10-Cy5 wt+3-Cy3 Set 2 (dye swap): wt+10-Cy3 wt+3-Cy5 Set 3: wt+10-Cy5 wt+3-Cy3 Set 3 (dye swap): wt+10-Cy3 wt+3-Cy5 GSM63403, GSM63404, GSM63405, GSM63406, GSM63407, GSM63408 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+15-Cy5 wt+3-Cy3 Set 1 (dye swap): wt+15-Cy3 wt+3-Cy5 Set 2: wt+15-Cy5 wt+3-Cy3 Set 2 (dye swap): wt+15-Cy3 wt+3-Cy5 Set 3: wt+15-Cy5 wt+3-Cy3 Set 3 (dye swap): wt+15-Cy3 wt+3-Cy5 GSM63409, GSM63410, GSM63411, GSM63412, GSM63413, GSM63414 Data for six replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+20-Cy5 wt+3-Cy3 Set 1 (dye swap): wt+20-Cy3 wt+3-Cy5 Set 2: wt+20-Cy5 wt+3-Cy3 Set 2 (dye swap): wt+20-Cy3 wt+3-Cy5 Set 3: wt+20-Cy5 wt+3-Cy3 Set 3 (dye swap): wt+20-Cy3 wt+3-Cy5 GSM63415, GSM63416, GSM63417, GSM63418, GSM63419, GSM63420
2010-07-01 | E-GEOD-2901 | biostudies-arrayexpress
Project description:We constructed cDNA library from 0-10 day post anthesis cotton fibers when fiber cells begin to initiate and elongate throughout this peroid. We randomly sequenced over 95,000 ESTs from this library, and with request of upland cotton ESTs from other laboratories, we acquired a gene pool of more than 30,000 UniESTs. The cotton UniESTs were then PCR-amplified and printed onto microarray. This array is comprised of about 29,000 high-quality cotton cDNAs (each sequence length>400bp, average length > 600bp) and external controls. Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt0-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 wt0-Cy5 Set 2: wt0-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 wt0-Cy5 Set 3: wt0-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 wt0-Cy5 Set 4: wt0-Cy5 wt-3-Cy3 Set 4 (dye swap): wt-3-Cy3 wt0-Cy5 GSM209619, GSM209620, GSM209621, GSM209622, GSM209623, GSM209624, GSM209625, GSM209626, Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+1-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 wt+1-Cy5 Set 2: wt+1-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 wt+1-Cy5 Set 3: wt+1-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 wt+1-Cy5 Set 4: wt+1-Cy5 wt-3-Cy3 Set 4 (dye swap): wt-3-Cy3 wt+1-Cy5 GSM209627, GSM209628, GSM209629, GSM209630, GSM209631, GSM209632, GSM209633, GSM209634, Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+2-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 wt+2-Cy5 Set 2: wt+2-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 wt+2-Cy5 Set 3: wt+2-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 wt+2-Cy5 Set 4: wt+2-Cy5 wt-3-Cy3 Set 4 (dye swap): wt-3-Cy3 wt+2-Cy5 GSM209635, GSM209636, GSM209637, GSM209638, GSM209639, GSM209640, GSM209641, GSM209642, Data for 8 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: wt+3-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 wt+3-Cy5 Set 2: wt+3-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 wt+3-Cy5 Set 3: wt+3-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 wt+3-Cy5 Set 4: wt+3-Cy5 wt-3-Cy3 Set 4 (dye swap): wt-3-Cy3 wt+3-Cy5 GSM209643, GSM209644, GSM209645, GSM209646, GSM209647, GSM209648, GSM209649, GSM209650, Data for 6 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: fl+3-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 fl+3-Cy5 Set 2: fl+3-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 fl+3-Cy5 Set 3: fl+3-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 fl+3-Cy5 GSM209455, GSM209456, GSM209608, GSM209609, GSM209610, GSM209612, Data for 6 replicate experiments performed with biologically independent samples. Cy3 and Cy5 dyes were used as follows: Set 1: fl-3-Cy5 wt-3-Cy3 Set 1 (dye swap): wt-3-Cy3 fl-3-Cy5 Set 2: fl-3-Cy5 wt-3-Cy3 Set 2 (dye swap): wt-3-Cy3 fl-3-Cy5 Set 3: fl-3-Cy5 wt-3-Cy3 Set 3 (dye swap): wt-3-Cy3 fl-3-Cy5 GSM209613, GSM209614, GSM209615, GSM209616, GSM209617, GSM209618
2008-04-01 | E-GEOD-8443 | biostudies-arrayexpress
Project description:Histone methylation is one of the most significant epigenetic modifications in plants because it is responsible for regulating and controlling the expression of many important functional genes. Although there have been many studies concerning histone methylation, its specific functions and mechanisms in cotton fiber initiation remain unclear. To determine if the H3K27me3 modification had regulated cotton fiber initiation in upland cotton (Gossypium hirsutum), we performed a comparative analysis on the cleavage under targets and tagmentation (CUT&Tag) between the wild-type cultivar Xu142 and its fibreless mutant Xu142 fl. This research was carried out three time points during the initiation period of cotton fibers, which were -1, 0 and 1 days post-anthesis (DPA). We also used the H3K27me3 antibody in Xu142 and Xu142 fl to examine the differential deposition of H3K27me3. Combing these results with RNA-seq analysis, we discovered a negative correlation between transcription and H3K27me3. We identified 336 genes with opposing trends in H3K27me3 deposition and expression. These genes are involved in various metabolic pathways, including fatty acid biosynthesis, galactose metabolism, plant hormone signal transduction, plant-pathogen interaction, glycolysis-/-gluconeogenesis, phenylpropanoid biosynthesis, and cutin, suberin and wax biosynthesis. Further physiological research revealed that in vitro ovule culture with an RDS 3434 inhibitor could alters the level of H3K27me3 in ovules, thus influencing the process of cotton fiber development. Taken together, these results indicate that H3K27me3 plays an important role in the fiber initiation process of cotton, and demonstrates the H3K27me3 network involved in fiber initiation in cotton ovules. The study also provides a foundation for the future study of genes involved in fiber development.
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2015-05-01 | GSE66251 | GEO
Project description:Transcriptional profiling of cotton ovule cells. Comparison of expression of genes in cultured ovules (-1 DPA) treated with or without phytohormones (5um IAA and 1um GA) and over a time-course from 0 to 12 hours. The phytohormone incubation periods for unfertilized ovules were 0, 1, 3, 6, and 12 hours (H) with two biological replicates at each time-point.
2015-05-01 | E-GEOD-66251 | biostudies-arrayexpress
Project description:Through genome-wide comparative transcriptome analysis of 63 samples, the current study identified nine key genes and pathways associated with biological process of yield heterosis in upland cotton. Our results and data resources provide novel insights and will be useful for dissecting the molecular mechanism of yield heterosis in cotton.
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Project description:Gossypium hirsutum strain:upland cotton Raw sequence reads
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