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RNA Sequencing Identification of MC38-P and Its Liver Metastatic Derivatives, MC38-LM3a and MC38-LM3b [Bulk RNA-seq]

ABSTRACT: Mouse-derived MC38 cells were subjected to in vivo selection to obtain colorectal cancer liver metastatic derivatives, MC38-LM3a and MC38-LM3b. Subsequently, their gene expression characteristics were identified through transcriptomic sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE290134 | GEO | 2025/09/10

REPOSITORIES: GEO

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RNA Sequencing Identification of MC38-P and Its Liver Metastatic Derivatives, MC38-LM3a and MC38-LM3b [Bulk RNA-seq]

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Single cell RNA-seq profiling of T cells isolated from MC38 mouse tumors

Project description:We investigate the single-cell landscape of the inflammatory mouse tumor model MC38, a C57BL/6 tumor cell line derived from colon adenocarcinoma. MC38 (diluted in HBSS and matrigel) was inoculated in the right unilateral flank (in the border of positions B2 and B3) of C57BL/6 mice (ref Study 16-3384 AV). Tumors were taken one day after group-out (average 150-250 mm3 at day 0), approximately 14-19 days. Tissues were dissociated and flow sorted accordingly to obtain the following groups for 10x Chromium 5' Gene Expression Profiling. Our results indicate that the degree of clonal expansion is correlated with expression of T cell exhaustion markers, and that T cells with strong exhaustion phenotype also express high levels of activation markers, such as interferon gamma.

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