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Comprehensive profiling of N6-methyladnosine (m6A) readouts reveals novel m6A readers that regulate human embryonic stem cell differentiation [GLORI-seq]

ABSTRACT: GLORI-Seq is an antibody-independent sequencing, which can detect m6A sites at single-base. The core of GLORI-Seq is to find a catalytic system of glyoxal and nitrite through chemical reaction combination screening, which efficiently deaminate unmethylated adenosine A to form inosine (A-to-I, > 98%). Inosine is read to guanosine (G) in the sequencing process, forming A-to-G conversion. GLORI-Seq enables single base detection of single base m6A by detecting the proportion of “A” in the sequenced read sequence.

ORGANISM(S): Homo sapiens

PROVIDER: GSE290408 | GEO | 2026/02/09

REPOSITORIES: GEO

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