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PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [pbap2-g_KO_RNA-seq]

ABSTRACT: Plasmodium berghei transcriptomes of gametocyte-absent mutant parasites, the pbap2-g-knockout line (pbap2-g(-)), were analyzed from ring to trophozoite stages by high-throughput RNA sequencing (RNA-seq) analysis.

ORGANISM(S): Plasmodium berghei ANKA

PROVIDER: GSE290541 | GEO | 2025/07/01

REPOSITORIES: GEO

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PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_RNA-seq]

Project description:Plasmodium berghei transcriptomes of pbap2-tr-DiCre, a parasite line in which pbap2-tr is disrupted by DiCre-mediated recombination in the presence of rapamycin, were analyzed at the trophozoite stage.

2025-07-01 | GSE290545 | GEO

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_ChIP-seq]

Project description:To investigate the genome-wide binding sites of PbAP2-TR and PbMORC in the asexual blood stage of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-TR or GFP-fused PbMORC were generated from the Cas9-expressing parasite (PbCas9), and pbap2-g was disrupted in these parasites (PbAP2-TR::GFPpbap2-g(-) and PbMORC::GFPpbap2-g(-)). Whole blood was harvested from mice infected with these transgenic parasites at 12 h post infection,and the parasites were then subjected to ChIP-seq experiments using anti-GFP antibody. Genome-wide binding sites of PbAP2-TR were determined from the sequence data.

2025-07-01 | GSE290542 | GEO

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_DIP-seq]

Project description:To investigate the DNA-binding property of AP2 domains of PbAP2-TR, DNA immunoprecipitation followed by high-throughput sequencing (DIP-seq) analysis was performed. Recombinant AP2 domains 1–2 or AP2 domain 3 fused with maltose-binding protein (MBP) were mixed with the P. berghei genomic DNA fragmented via sonication, and protein-DNA complex was harvested using amylose resin. The obtained DNA fragments were sequenced via the next generation sequencing.

2025-07-01 | GSE290544 | GEO

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_DIP-seq]

Project description:PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_DIP-seq]

| PRJNA1228587 | ENA

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_ChIP-seq]

Project description:PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_ChIP-seq]

| PRJNA1228589 | ENA

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [pbap2-g_KO_RNA-seq]

Project description:PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [pbap2-g_KO_RNA-seq]

| PRJNA1228596 | ENA

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_RNA-seq]

Project description:PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_RNA-seq]

| PRJNA1228597 | ENA

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [RNA-seq]

Project description:Plasmodium berghei transcriptomes of pbap2-fg2 and ap2r-2 knockout parasites [pbap2-fg2(-) and ap2r-2(-), respectively] were analyzed at the gametocyte stage.

2023-02-02 | GSE213773 | GEO

ChIP-seq on PfAP2-G2 in trophozoite and gametocyte stages of parasites.

Project description:Differentiation from asexual blood stages to sexual gametocytes is required for transmission of malaria parasites from the human host to mosquitos, where sexual fertilization occurs to complete the lifecycle. Although preventing gametocyte development would block parasite transmission, the molecular mechanisms underlying sexual commitment and gametocyte maturation are still relatively unknown. Previous studies identified an ApiAP2 protein, AP2-G2, which plays a critical role in gametocyte maturation in rodent malaria parasite Plasmodium berghei by acting as the repressor of asexual stage genes in gametocytes. In this work we characterize the P. falciparum orthologue (PF3D7_1408200) of PbAP2-G2 and report that it plays a critical role in maturation of gametocytes. Disruption of pfap2-g2 did not obstruct commitment to sexual development but the majority of parasites were unable to develop normally beyond stage III gametocytes. In asexual stages PfAP2-G2 binds to the promoters of wide variety of genes expressed in diverse range of parasite’s life cycle stages including gametocytes, mosquito lifecycle stages early ring stage genes and genes encoding for proteins involved in egress and invasion. Surprisingly, we also identify binding of PfAP2-G2 in the gene body of almost 3000 genes. We could also show that PfAP2-G2 interacts with chromatin remodeling proteins and another ApiAP2 protein (PF3D7_1139300) whose motif is also overrepresented in the ChIP-seq data. Overall this work suggests that PfAP2-G2 is a transcriptional regulator that regulates genes possibly by recruiting additional transcription factors and chromatin remodeling machinery and plays a critical role in the development of malaria parasites as they transition from the asexual stage to gametocytes.

2021-02-16 | GSE157753 | GEO

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

Project description:To investigate the genome-wide binding sites of the transcriptional regulators, PbAP2-FG2 and AP2R-2 in female gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-FG2 (PbAP2-FG2::GFP) or GFP-fused AP2R-2 (AP2R-2::GFP) were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data.

2023-02-02 | GSE213775 | GEO

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