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Development of AptCD4-LNT for selective mobilization and activation CD4+ T cells to enhance anti-tumor immunity in triple-negative breast cancer

ABSTRACT: CD8+ T cells are well-established primary effectors in anti-tumor immunity, but increasing evidence underscores the critical and multifaceted role of CD4+ T cells, which can function both as helper cells and direct tumor killers. However, the clinical potential of CD4+ T cells has been limited by poor tumor infiltration and the challenges posed by an immunosuppressive tumor microenvironment. To address these limitations, we developed a CD4 aptamer-engineered liquid nitrogen-treated cancer cell system (AptCD4-LNT) designed to selectively activate and recruit CD4+ T cells for improved anti-tumor immunity. This system leverages the CD4 aptamer's specificity for CD4+ T cells and the homing properties of LNT cells to drive targeted infiltration and activation of CD4+ T within the tumor microenvironment. Activated CD4+ T cells, in turn, enhance the infiltration and activity of NK cells, B cells, and dendritic cells, reshaping the immune landscape and potentiating anti-tumor responses in triple-negative breast cancer. Our findings highlight the pivotal role of CD4+ T cells in TNBC immunotherapy and present a promising strategy for advancing CD4-targeted treatments in clinical settings.

ORGANISM(S): Mus musculus

PROVIDER: GSE290903 | GEO | 2025/09/20

REPOSITORIES: GEO

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