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Multiplexage : dual-target hybridization microarray experiment-New developments towards a genetic and diagnosis use of plant mircoarrays

ABSTRACT: gnp06-01_agriarray - multiplexage : dual-target hybridization microarray experiment - Several factors affect today a more extended use of microarrays for research and as a diagnosis tool. These factors are the experimental cost, the reproducibility of the measurements and the format of the analyses. This project aims at bringing solutions in these three domains by optimizing multiplexed analyses in order to reduce cost and enhance the number of samples treated simultaneously; by proposing new couples flurophores / slide surface in order to increase the signal precision and by developing new applications such as Comparative Genomic Hybridization (GGH) or Promoter Arrays to bring the plant microarray beyond transcriptome analyses. Altogether, these approaches will allow us to construct an optimized diagnostic tool based on 96 well microplate microarrays. This multidisciplinary project bring together biologists, chemists, physicists and mathematicians to develop innovative solutions for future application of DNA microarrays. - Dual-target hybridization microarray experiment with doble factors comparison : two mutants and its wild-types at different times. The aim of this part is to compare the classical design with the new design with a third dye.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE29104 | GEO | 2011/12/08

SECONDARY ACCESSION(S): PRJNA140363

REPOSITORIES: GEO

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nitrogen starvation -New developments towards a genetic and diagnosis use of plant mircoarrays

Project description:gnp06-01_agriarray - nitrogen starvation - Several factors affect today a more extended use of microarrays for research and as a diagnosis tool. These factors are the experimental cost, the reproducibility of the measurements and the format of the analyses. This project aims at bringing solutions in these three domains by optimizing multiplexed analyses in order to reduce cost and enhance the number of samples treated simultaneously; by proposing new couples flurophores / slide surface in order to increase the signal precision and by developing new applications such as Comparative Genomic Hybridization (GGH) or Promoter Arrays to bring the plant microarray beyond transcriptome analyses. Altogether, these approaches will allow us to construct an optimized diagnostic tool based on 96 well microplate microarrays. This multidisciplinary project bring together biologists, chemists, physicists and mathematicians to develop innovative solutions for future application of DNA microarrays. - Dual-target hybridization microarray experiment.

2011-12-08 | GSE29103 | GEO

nitrogen starvation -New developments towards a genetic and diagnosis use of plant mircoarrays

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multiplexage : dual-target hybridization microarray experiment-New developments towards a genetic and diagnosis use of plant mircoarrays

Project description:gnp06-01_agriarray - multiplexage : dual-target hybridization microarray experiment - Several factors affect today a more extended use of microarrays for research and as a diagnosis tool. These factors are the experimental cost, the reproducibility of the measurements and the format of the analyses. This project aims at bringing solutions in these three domains by optimizing multiplexed analyses in order to reduce cost and enhance the number of samples treated simultaneously; by proposing new couples flurophores / slide surface in order to increase the signal precision and by developing new applications such as Comparative Genomic Hybridization (GGH) or Promoter Arrays to bring the plant microarray beyond transcriptome analyses. Altogether, these approaches will allow us to construct an optimized diagnostic tool based on 96 well microplate microarrays. This multidisciplinary project bring together biologists, chemists, physicists and mathematicians to develop innovative solutions for future application of DNA microarrays. - Dual-target hybridization microarray experiment with doble factors comparison : two mutants and its wild-types at different times. The aim of this part is to compare the classical design with the new design with a third dye. 47 dye-swap - genotype and ecotype comparison

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