Transcriptomics

Dataset Information

40

Bglu3 congenic mice - gene expression in liver


ABSTRACT: A congenic line was constructed by introgressing a C3H chromosome 1 region harboring Bglu3 into C57BL/6 apoE-/- background. RNA was extracted from liver using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the liver of Bglu3 congenic mice and C57BL/6 apoE-deficient mouse strains fed a western diet. Overall design: 2 groups of mice were studied: C57BL/6 apoE-/- control mice and Bglu3 congenic mice. After fed a Western diet for 12 weeks, liver was harvested and total RNA prepared.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: qian wang  

PROVIDER: GSE29151 | GEO | 2011-05-11

SECONDARY ACCESSION(S): PRJNA140005

REPOSITORIES: GEO

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