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Gene expression profiling of Mycobacterium tuberculosis H37Rv in response to compound CDRI-5g

ABSTRACT: Gene expression profiling of Mycobacterium tuberculosis H37Rv in response to compound CDRI-5g (Saquib, M. et al. Eur. J. Med. Chem. 46 (2011) 2217-2223) was studied by administration of compound at 10 µg/ml in broth culture. RNA was extracted at two time points 12 h and 24 h of growth from replicating phase and compound added culture. Replicating phase culture was incubated for the same time and used as a control. Experiment was repeated three times and for each time points triplicate culture was pelleted to isolate total RNA using Qiagen RNA isolation kit.

ORGANISM(S): Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv

PROVIDER: GSE29166 | GEO | 2011/09/01

SECONDARY ACCESSION(S): PRJNA140087

REPOSITORIES: GEO

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Gene expression profiling of Mycobacterium tuberculosis H37Rv in response to compound CDRI-5g

Project description:Gene expression profiling of Mycobacterium tuberculosis H37Rv in response to compound CDRI-5g (Saquib, M. et al. Eur. J. Med. Chem. 46 (2011) 2217-2223) was studied by administration of compound at 10 µg/ml in broth culture. RNA was extracted at two time points 12 h and 24 h of growth from replicating phase and compound added culture. Replicating phase culture was incubated for the same time and used as a control. Experiment was repeated three times and for each time points triplicate culture was pelleted to isolate total RNA using Qiagen RNA isolation kit. One-color experiment,Genotypic Technology designed Custom Mycobacterium tuberculosis H37Rv Whole Genome 8x15k GE Microarray (AMADID-019943)

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Transciptional profile of a M. smegmatis mc2155 ÎsigF strain under standard growth conditions

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