Il7rKO Single Cell

ABSTRACT: The immune system must distinguish pathogens from innocuous dietary antigens, but the precise mechanisms and cellular actors involved are unclear. Here, we demonstrate that RORγt-lineage antigen-presenting cells (APCs) expressing the Autoimmune Regulator (Aire) gene – referred to as RORγt eTACs – are required for oral tolerance. Using lineage tracing and single-cell sequencing, we show that RORγt eTACs are of a myeloid-derived lineage and establish consensus identities for all RORγt-lineage APCs. We found that RORγt eTACs, but not type 3 innate lymphoid cells, are necessary and sufficient for oral tolerance induction. Upon depletion of RORγt eTACs, mice fail to develop food-specific Tregs and display severe delayed-type hypersensitivity responses. These findings establish RORγt eTACs as critical mediators of oral tolerance and suggest novel targets to modulate immune tolerance.

ORGANISM(S): Mus musculus

PROVIDER: GSE291696 | GEO | 2025/04/15

REPOSITORIES: GEO