Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes

ABSTRACT: mRNA expression profiles from interscapular brown adipose (iBAT) tissue male ATF4 BAT KO mice and their WT counterparts after 12 weeks of high fat diet.

ORGANISM(S): Mus musculus

PROVIDER: GSE291759 | GEO | 2025/07/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes.

Project description:mRNA expression profiles from interscapular brown adipose (iBAT) tissue male ATF4 BAT KO mice and their WT counterparts after 3 days of cold exposure.

2024-01-24 | GSE227060 | GEO

Transcriptome analysis in brown adipose tissue of mice lacking the mitochondrial protein optic atrophy 1 (OPA1) in thermogenic adipocytes

Project description:mRNA expression profiles from interscapular brown adipose (iBAT) tissue of 7-week-old OPA1 BAT KO mice and their WT counterparts.

2023-10-10 | GSE218907 | GEO

Chaperone-mediated autophagy controls brown adipose tissue 2 thermogenic activity

Project description:The activity of brown fat, a tissue mediating non-shivering thermogenesis, is associated with protection against obesity, diabetes, and cardiovascular disease. In response to thermogenic stimuli, brown adipose tissue (BAT) increases its activity through extensive cellular and tissue plasticity. While macroautophagy is typically inhibited during BAT thermogenic activation, we found that chaperone-mediated autophagy (CMA), a selective type of autophagy, is instead induced. CMA induction enhances both basal and cAMP-stimulated activity of brown adipocytes, as evidenced by increased expression of thermogenesis-related genes, enhanced release of brown adipokines, elevated cellular oxidative activity, and increased lipolysis. In aging, when BAT activity declines, LAMP2A—the limiting CMA component—is downregulated in BAT. However, pharmacological activation of CMA restores BAT activity in aged mice. To investigate the consequences of this CMA failure in BAT, we generated brown adipocytes knock down for LAMP2A and using comparative proteomics identified that CMA regulates levels of proteins involved in BAT thermogenic and metabolic activity. We demonstrate that selective CMA blockade in interscapular BAT in mice leads to reduced energy expenditure, increased triglyceridemia, lower expression of thermogenic markers and BAT-secretory functions, and to accumulation of thermogenesis repressors upon thermogenic activation. Overall, these findings support the essential role of CMA in BAT function and adaptation to thermogenic activation. CMA facilitates the timely degradation of thermogenic repressors, thereby promoting adaptive enhancement of thermogenic activity.

2025-09-30 | PXD062807 | Pride

Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes

Project description:Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes

| PRJNA1235202 | ENA

Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes.

Project description:Transcriptome analysis in brown adipose tissue of mice lacking ATF4 in thermogenic adipocytes.

| PRJNA942971 | ENA

RNA-seq comparison of primary mouse classical brown, beige, and white adipocytes

Project description:Classical brown adipocytes in interscapular BAT (Myf-5 derived) and inducible beige cells in WAT (non-Myf-5 derived) have distinct developmental origins, although both cell types have morphological and biochemical characteristics of brown fat such as the expression of UCP1. This raises an important question as to how similar the two types of brown adipocytes are at molecular and functional levels. To this end, we employed RNA-seq to systematically determine the transcriptional signatures unique to each cell type.

2015-03-16 | E-MTAB-2624 | biostudies-arrayexpress

Transcriptome profiling of brown adipose tissue during cold exposure

Project description:We applied a deep-sequencing based method – digital gene expression profiling (DGEP), to investigate gene expression in interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) in acute cold exposure

2014-12-19 | GSE63031 | GEO

The acute cold response of brown adipose tissue analyzed by RNA-seq

2015-11-25 | GSE70437 | GEO

The acute cold response of brown adipose tissue analyzed by RNA-seq

Project description:We applied RNA sequencing (RNA-seq) to map the global changes in gene expression of interscapular brown adipose tissue (iBAT) of mice subjected to acute cold exposure for 3 days. Here we find extensive changes in the iBAT transcriptome in response to cold with a prominent induction of genes associated to lipid-related metabolic processes. RNA-seq of poly-A enriched RNA isolated from brown adipose tissue of 5 mice housed at room temperature (22Â°C) and 5 mice exposed to cold (4Â°C) for 3 days.

2015-11-25 | E-GEOD-70437 | biostudies-arrayexpress

RNA-seq of male and female mouse brown adipose tissue (BAT) transcriptome

Project description:The interscapular brown adipose tissue (BAT) depots of adult male and female C57BL/6J mice, housed at 22 °C, were analyzed to identify sex differences in the BAT transcriptome at basal housing conditions.

2021-06-16 | E-MTAB-8717 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data