Unknown

Dataset Information

51

Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)


ABSTRACT: Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human ChIP-seq portion of this dataset on leukemia cell lines. Overall design: Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008), CEBPA (SC-9314) or H3K4me1 (Abcam ab8895).

INSTRUMENT(S): Illumina Genome Analyzer II (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Teresa Venezia Bowman 

PROVIDER: GSE29195 | GEO |

SECONDARY ACCESSION(S): PRJNA142955

REPOSITORIES: GEO

Similar Datasets

2011-10-07 | E-GEOD-29195 | ArrayExpress
2011-10-07 | E-GEOD-29194 | ArrayExpress
2011-10-07 | E-GEOD-29193 | ArrayExpress
2010-05-13 | E-TABM-828 | ArrayExpress
2012-03-14 | E-GEOD-33850 | ArrayExpress
2012-06-22 | E-MTAB-941 | ArrayExpress
2011-07-25 | E-GEOD-30890 | ArrayExpress
2012-02-09 | E-GEOD-29180 | ArrayExpress
2012-07-09 | E-GEOD-39179 | ArrayExpress
2019-03-21 | PXD012930 | Pride