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Differential gene expression in immortal DF-1 chicken embryo fibroblast cell line

ABSTRACT: Transcriptional profiling of chicken embryo fibroblast (CEF) cells comparing CEF derived immortal chicken embryo fibroblast cell line (DF-1). Goal was to determine differentially expressed genes of CEF which were changed responding DF-1.

ORGANISM(S): Gallus gallus

PROVIDER: GSE29257 | GEO | 2011/05/12

SECONDARY ACCESSION(S): PRJNA140257

REPOSITORIES: GEO

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Differential gene expression in immortal DF-1 chicken embryo fibroblast cell line

Project description:Transcriptional profiling of chicken embryo fibroblast (CEF) cells comparing CEF derived immortal chicken embryo fibroblast cell line (DF-1). Goal was to determine differentially expressed genes of CEF which were changed responding DF-1. Two-condition experiment, CEF vs. DF-1. Biological replicates: 1 control CEF sample, 1 DF-1 experimental sample.

2011-05-12 | E-GEOD-29257 | biostudies-arrayexpress

Genome-wide comparison of Chicken Embryo Fibroblasts (CEF) and DF-1 in response to recombinant chicken chIFN-Î± and infection with Infectious Bursal Disease Virus (IBDV)

Project description:The spontaneously immortalised chicken DF-1 cell line is rapidly replacing its progenitor primary chicken embryo fibroblasts (CEF) in studies on avian viruses but no comprehensive study has as yet been reported comparing their immune phenotype. We conducted microarray analysis of the DF-1 and CEF, in both normal and stimulated conditions using recombinant chicken chIFN-Î± and the CEF-adapted infectious bursal disease virus vaccine strain PBG98.

2016-01-15 | E-MTAB-4028 | biostudies-arrayexpress

Microarray analysis in senescent chicken embryo fibroblast

2011-05-12 | GSE29258 | GEO

Microarray analysis in senescent chicken embryo fibroblast

Project description:Transcriptional profiling of healthy chicken embryo fibroblast (CEF, P4) cells comparing senescent chicken embryo fibroblast (CEF, P18) cells. Goal was to identify differentially expressed genes comparing between healthy (P4) and senescent (P18) CEF cells. Two-condition experiment, healthy CEF (P4) vs. senescence CEF (P18). Biological replicates: 1 control CEF (P4) sample, 1 CEF (P18) experimental sample.

2011-05-12 | E-GEOD-29258 | biostudies-arrayexpress

Gallus gallus

Project description:Differential gene expression in immortal DF-1 chicken embryo fibroblast cell line

| PRJNA140257 | ENA

Single cell RNA-seq in chicken's lung and DF-1 cell line

Project description:Newcastle disease virus (NDV) is an avian paramyxovirus that causes major economic losses to the poultry industry around the world, with NDV pathogenicity varying due to strain virulence differences. However, the impact of intracellular viral replication and the heterogeneity of host responses among cell types are unknown. Here, we investigated the heterogeneity of lung tissue cells in response to NDV infection in vivo and that of chicken embryo fibroblast cell line DF-1 to NDV infection in vitro using single-cell RNA sequencing.

2022-12-31 | GSE202128 | GEO

Gene profiling of v-Src transformed primary chicken cells

Project description:Cell transformation by the Src tyrosine kinase is characterized by extensive changes in gene expression. To describe these changes, investigators have relied extensively on the study of immortalized rodent cell lines or heterogeneous tumor samples that limit the identification of differentially expressed genes or may not represent the full spectrum of biological processes regulated during transformation. In this study, we took advantage of transformation-deficient and temperature sensitive mutants of the Rous sarcoma virus to characterize the patterns of gene expression in two types of primary cells, namely chicken embryo fibroblasts (CEF) and chicken neuro-retinal (CNR) cells. Keywords: viral transformation of primary cells, transformation, transformation deficient mutant, temperature sensitive mutant, v-Src Chicken embryo fibroblasts (CEF) were infected with the wild-type strain Schmidt-Ruppin A RSV or non-transforming strain NY315 RSV or the non-transforming control virus RCASBP(A) to assess genes involved in v-Src-dependent transformation of CEF. Chicken embryo fibroblasts (CEF) were infected with the temperature sensitve strain NY72-4 RSV and cultured either at non-permissive temperature (41.5M-KM-^ZC) or permissive temperature (37M-KM-^ZC) to assess genes involved in v-Src-dependent transformation of CEF. Chicken neuroretina cells (CNR) were infected with the temperature sensitve strain NY72-4 RSV and cultured either at non-permissive temperature (41.5M-KM-^ZC) or permissive temperature (37M-KM-^ZC) to assess genes involved in v-Src-dependent transformation of CNR and compared to CEF.

2010-02-21 | E-GEOD-14489 | biostudies-arrayexpress

Comparative RNAseq analysis of CSF1-stimulated chicken bone marrow-derived macrophages and embryonic RNA

Project description:RNAs were analysed from a pool of day 7 chicken embryos, the DF-1 chicken fibroblast cell line and chicken bone marrow-derived macrophages (stimulated with CSF-1).

2015-01-19 | E-MTAB-3048 | biostudies-arrayexpress

Chicken IRF7 knockout by CRISPR-Cas9 in DF-1 and in vitro H6N2 infection followed by RNA-seq

Project description:To elucidate the functional role of chicken IRF7 against avian influenza virus (AIV) infection, we generated inducible IRF7 knockout DF-1 cell lines and performed in vitro infection using low pathogenic AIVs (LPAIVs) followed by RNA-seq.

2018-10-01 | GSE119590 | GEO

Project description:df

| PRJEB32301 | ENA

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