Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE292607 | GEO | 2026/06/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [scRNAseq]

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor We characterized mPB CD34+ cell population dynamics (n=2 donors) by single cell RNA sequencing (scRNAseq) during 8 days of expansion culture using commercial serum-free media (Miltenyi Bioscience) in the presence of the epigenetic modifier UM171 and early acting cytokines (SFT6: stem cell factor, 100ng/mL; flt3 ligand, 100ng/mL; thrombopoietin, 50ng/mL; interleukin 6, 50ng/mL). Cultured cells were transduced with a lentiviral vector expressing a marker gene, to simulate a gene therapy context. CD34+ cells from donor 1 were analyzed before culture, on day 4 and on day 8 of expansion. For donor 2, in addition to the CD34+ bulk expansion culture, part of the CD34+ cells were FACS-sorted into subpopulations enriched in primitive HSPC (CD34+CD38-), committed granulocyte-monocyte progenitors (GMP) or megakaryocyte-erythrocyte progenitors (MEP), and subsequently expanded for 7 days.

2026-06-10 | GSE292606 | GEO

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [bulkRNAseq]

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor. We characterized mPB CD34+ drug products and their ex-vivo expanded samples (4 and 7 days) cell populations (n=2 patients from 10.1056/NEJMoa2106596) and transduction efficency by single cell RNA sequencing (scRNAseq) using commercial serum-free media in the presence of the epigenetic modifier UM171 and early acting cytokines (DP: refers to 10.1056/NEJMoa2106596; for cultured samples at day4 and day 7: SFT6: stem cell factor, 100ng/mL; flt3 ligand, 50ng/mL). Cells of DP and its expanded samples were transduced with a lentiviral vector expressing the IDUA gene. WPRE transcript quantification was used as surrogate of LV IDUA expression to evaluated transduction efficiency.

2026-06-10 | GSE292605 | GEO

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [MPS_scRNAseq]

2026-06-10 | GSE292604 | GEO

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor

| PRJNA1240289 | ENA

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [MPS_scRNAseq]

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [MPS_scRNAseq]

| PRJNA1240294 | ENA

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [bulkRNAseq]

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [bulkRNAseq]

| PRJNA1240295 | ENA

Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [scRNAseq]

Project description:Clonal analysis and transcriptional profiling of ex vivo expanded mobilized peripheral blood hematopoietic stem and progenitor [scRNAseq]

| PRJNA1240296 | ENA