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Effects of treatment with cyanidin-3-glucoside on mammary tumors arising from MMTV-PyVT mice

ABSTRACT: Cyanidin-3-glucoside (C3G) is one of the most prevalent anthocyanins among dietary polyphenols. Experimental studies suggest that C3G exhibits antioxidant, anti-inflammatory, and cytoprotective effects against various disorders induced by oxidative stress. We examined its in vivo anti-neoplastic properties using MMTV-PyVT transgenic mice, a well-validated model for the development and progression of multistage mammary carcinoma.

ORGANISM(S): Mus musculus

PROVIDER: GSE292678 | GEO | 2025/03/24

REPOSITORIES: GEO

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Effects of treatment with cyanidin-3-glucoside on mammary tumors arising from MMTV-PyVT mice

Project description:Effects of treatment with cyanidin-3-glucoside on mammary tumors arising from MMTV-PyVT mice

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Understanding the functional role of cyanidin-3-O-glucoside in mouse osteoblastic cell line MC3T3-E1 by RNA-seq analysis

Project description:Cyanidin-3-O-glucoside (C3G) is one of the most widely distributed anthocyanins and has the effect of reducing the risks of osteoporosis. However, the molecular mechanism by which C3G promotes bone formation remains incompletely understood. Our aim was to investigate the effect of C3G on gene expression profiles, during the proliferation and differentiation in MC3T3-E1, a kind of osteoblast cell line from mice, by RNA sequencing. The results identified 34 up-regulated and 17 down-regulated genes. We further performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes analyses (KEGG), and other bioinformatics methods to do further analysis of differentially expressed genes (DEGs). Analysis of sequencing data showed that these genes were highly enriched in functions related to Lysosomal and Glycolipid biosynthetic. Lastly, we used RT-qPCR to validate the differentially expressed mRNAs. The results showed that C3G increased the expression levels of Ly6a (Lymphocyte antigen 6 family member A), Atp6v0c (ATPase H+ transporting v0 subunit c), and decreased the expression levels of Cx3cl1 (C-X3-C motif chemokine ligand 1). This study suggested that C3G activated osteoblast via Glycosylphosphatidylinositol(GPI)-anchor biosynthesis and the Cx3cl1/Cx3cr1 signaling pathway.

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Quantitative phosphosproteomics revealed the mechanism of Cyanidin-3-O-glucoside ameliorating dextran sulfate sodium (DSS)-induced colitis

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Mechanisms research on Cyanidin-3-O-glucoside ameliorating dextran sulfate sodium (DSS)-induced colitis.

Project description:TMT proteomics for colonic mucosa in DSS-induced colitis mice with or without administrated Cyanidin-3-O-glucoside was used for discovery of differential expressed proteins.

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Comparative analysis of the effect of different diets on histone H3K4me3 distribution in mouse liver chromatin reveals the epigenetic efficacy of cyanidin-3-O-glucoside dietary intake

Project description:Background: Different diets result in significantly different phenotypes through metabolic and genomic reprogramming. Epigenetic marks, identified in humans and mouse models upon caloric restriction, high fat diet or the intake of specific bioactives, suggest that genomic reprogramming drives this reprogramming and mediates the effect of nutrition on health. Histone modifications encode the epigenetic signal that adapts genome functions to environmental conditions, including diets, by tuning the structure and properties of chromatin. So far, the effect of different diets on the genome-wide distribution of critical histone marks has not been determined Methods: We investigated by chromatin immunoprecipitation sequencing the distribution of the trimethylation of lysine 4 of histone H3 in the liver of mice fed for one year with five different diets including: chow containing corn powder as extra source of plant bioactives or specifically enriched with the cyanidin-3-O-glucoside, high fat enriched obesogenic and caloric restricted pro-longevity diets Conclusions: The comparison of the resulting histone mark profiles revealed functional food containing cyanidin determines broad effect.

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RNA analysis of Nr4a1 and Nr4a1-knockout mammary tumor cells from MMTV-PyVT transgenic mice

Project description:MMTV-PyVT Mammary tumors were harvested from PyVT-wild-type (WT), PyVT-Nr4a1 knockout (KO) littermates on a congenic FVB/NJ background,minced and incubated with collagenase type I digestion solution .The digested Mammary tumor cells were collected and maintained in DMEM medium containing 10% FBS for 24 hour.

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Understanding the functional role of cyanidin-3-O-glucoside in mouse osteoblastic cell line MC3T3-E1 by RNA-seq analysis

Project description:Understanding the functional role of cyanidin-3-O-glucoside in mouse osteoblastic cell line MC3T3-E1 by RNA-seq analysis

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Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice.

Project description:Among genetically engineered mouse models of breast cancer, MMTV-PyVT is a mouse strain in which the oncogenic polyoma virus middle T antigen is driven by the mouse mammary tumor virus promoter. The aim of the present study was to perform morphologic and genetic analyses of mammary tumors arising from MMTV-PyVT mice. To this end, mammary tumors were obtained at 6, 9, 12, and 16 weeks of age for histology and whole-mount analyses. We conducted whole-exome sequencing to identify constitutional and tumor-specific mutations, and genetic variants were identified using the GRCm38/mm10 mouse reference genome. Using hematoxylin and eosin analysis and whole-mount carmine alum staining, we demonstrated the progressive proliferation and invasion of mammary tumors. Frameshift insertions/deletions (indels) were noted in the Muc4. Mammary tumors showed small indels and nonsynonymous single-nucleotide variants but no somatic structural alterations or copy number variations. In summary, we validated MMTV-PyVT transgenic mice as a multistage model for mammary carcinoma development and progression. Our characterization may be used as a reference for guidance in future research.

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Expression data from Cyanidine (Cyanidine 3-glucoside)-treated human amnion epithelial cells (hAECs)

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Cyanidin-3-O-glucoside: Physical-Chemistry, Foodomics and Health Effects.

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| S-EPMC6273591 | biostudies-literature

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