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Gene expression patterns in response to GATA2 (WT and mutants) in HL-60 cells

ABSTRACT: GATA2 mutants were discovered in families predisposed to MDS/AML and in sporadic cases of CML-blast crisis. Promyelocytic HL-60 cells were transduced with lentiviral vectors that express GATA2 WT or T354M, 355delT or L359V mutants upon addition of 4-hydroxy tamoxifen (4HT). Microarrays were performed to identify GATA2 WT signatures and differences caused by these mutations.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29276 | GEO | 2011/08/20

SECONDARY ACCESSION(S): PRJNA139931

REPOSITORIES: GEO

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Gene expression patterns in response to GATA2 (WT and mutants) in HL-60 cells

Project description:GATA2 mutants were discovered in families predisposed to MDS/AML and in sporadic cases of CML-blast crisis. Promyelocytic HL-60 cells were transduced with lentiviral vectors that express GATA2 WT or T354M, 355delT or L359V mutants upon addition of 4-hydroxy tamoxifen (4HT). Microarrays were performed to identify GATA2 WT signatures and differences caused by these mutations. HL-60 cells transduced with GATA2 (WT, T354M, 355delT and L359V) lentiviruses were treated with 4HT for 24 hours and RNA isolated and gene expression measured on Affymetrix microarrays.

2011-08-19 | E-GEOD-29276 | biostudies-arrayexpress

GATA2 monoallelic expression underlies reduced penetrance in inherited GATA2- mutated MDS/AML

Project description:Although familial myelodysplasia and acute myeloid leukemia (MDS/AML) is rare, its true prevalence is likely underestimated due to wide variations in the age of onset, disease latency and outcome between and within families, with some mutation carriers remaining asymptomatic into late adulthood. Reduced penetrance is a notable feature of germline GATA2 p.Thr354Met pedigrees. In this study, we demonstrate that silencing of the wildtype (WT) GATA2 allele discriminates between symptomatic and asymptomatic carriers and is linked with allele-specific differences in DNA methylation and H3K4me3 promotor deposition, providing a molecular explanation for the clinical heterogeneity observed within a GATA2-mutated AML family.

2018-09-21 | GSE104570 | GEO

Mouse E11 WT and GATA2 heterozygous HSPCs

Project description:Transcriptome analysis from WT and Gata2 heterozygous mouse HSPCs at E11

2023-02-17 | E-MTAB-12571 | biostudies-arrayexpress

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GATA2 Deficiency

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Gene expression change in Gata2 conditional knockout mice adult renal collecting duct

Project description:We would like to know the gene expression pattern in absence of transcription factor GATA2 in adult renal collecting duct We used Gata2 flox::Pax8-rtTA::Tet-Cre to make a doxycycline induced Gata2 renal tubule cell specific knockout mice We performed microarray analyses using DBA-lectin and magnetic beads purifed collecting duct cells from WT (n=3) or Gata2 CKO mice (n=3) at 4-weeks after doxycycline induction

2013-11-19 | E-GEOD-52448 | biostudies-arrayexpress

RNAsequencing from mouse E11 WT and Gata2 heterozygous Pre1-, Pre2- and pro-HSCs

Project description:Transcriptional comparison of mouse E11 WT and Gata2 heterozygous Pre1-, Pre2- and pro-HSCs

2023-02-16 | E-MTAB-12570 | biostudies-arrayexpress

GATA2 knockout study to investigated the role of GATA2 in human hematopoiesis

Project description:We generated homozygous GATA2 knockout human embryonic stem cells (GATA2-/- hESCs) and analyzed their blood differentiation potential. Paritcularly at the hemogenic endothelium (HE) stage and hematopoietic progenitor cell (HPC) stage. Our result revealed that GATA2-/- hESCs displayed attenuated generation of CD34+CD43+ HPCs, due to the impairment of endothelial to hematopoietic transition (EHT). However, GATA2-/- hESCs retained the potential to generate erythroblasts, macrophages, but never granulocytes. Through RNA-Seq and further rescue experiment, we further identified that SPI1 was responsible for the defect of GATA2-/- hESCs in generation of CD34+CD43+ HPCs and granulocytes.

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Gata2 rescue assay

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GATA2 in human vascular endothelial cells

Project description:GATA2 is well recognized as a key transcription factor and regulator of cell type specificity and differentiation. Here, we carried out comparative chromatin immunoprecipitation with comprehensive sequencing (ChIP-seq) to determine genome-wide occupancy of GATA2 in endothelial cells and erythroids, and compared the occupancy to the respective gene expression profile in each cell type. Although GATA2 was commonly expressed in both cell types, different GATA2 bindings and distinct cell specific gene expressions were observed. By using the ChIP-seq with epigenetic histone modifications and chromatin conformation capture assays; we elucidated the mechanistic regulation of endothelial-specific GATA2 mediated endomucin gene expression, that was regulated by the endothelial-specific chromatin loop with a GATA2 associated distal enhancer and core promoter. Knockdown of endomucin markedly attenuated endothelial cell growth, migration and tube formation. Moreover, abrogation of GATA2 in endothelium demonstrated not only a reduction of endothelial specific markers, but also induction of mesenchymal transition promoting gene expression. Our findings provide new insights into the correlation of endothelial expressed GATA2 binding, epigenetic modification, and the determination of endothelial cell specificity. Total 4 samples were derived from duplicate or triplicate arrays in HMVEC or K562 cells. Then, HMVEC were transfected with si-RNA against GATA2 in biological duplicate. Overall, 6 samples were uploaded.

2011-06-13 | E-GEOD-28304 | biostudies-arrayexpress

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