Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of xenograft tumor in control group and C26 group

ABSTRACT: We performed RNA-sequencing (RNA-seq) with tumor tissues of mice MC38 xenograft models treated with C26.

ORGANISM(S): Mus musculus

PROVIDER: GSE292864 | GEO | 2025/06/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq of xenograft tumor in control group and C26 group

Project description:RNA-seq of xenograft tumor in control group and C26 group

| PRJNA1241772 | ENA

Exosomal miRNAs differences analysis between C26 and MC38

Project description:Exosomal miRNAs differences analysis between C26 and MC38

| PRJNA724681 | ENA

Leukemia Inhibitory Factor in C26 Cancer Cachexia

Project description:Cachexia is an exacerbating event in many types of cancer that is strongly associated with a poor prognosis. We have identified cytokine, signaling and transcription factors that are required for cachexia in the mouse C26 colon carcinoma model of cancer. C2C12 myotubes treated with conditioned medium from C26 cancer cells induced atrophy and activated a STAT-dependent reporter gene but not reporter genes dependent on SMAD, FOXO, C/EBP, NF-ĸB, or AP-1. Of the gp130 family members IL-11, IL-6, oncostatin M (OSM), and leukemia inhibitory factor (LIF), only OSM and LIF were sufficient to activate the STAT reporter in myotubes. A LIF blocking antibody abolished C26 CM-induced STAT reporter activation STAT3 phosphorylation and myotube atrophy, but blocking antibodies to IL-6 or OSM did not. JAK2 inhibitors also blocked the C26 CM-induced STAT reporter activation, STAT3 phosphorylation, and atrophy in myotubes. LIF at levels found in the C26 CM was sufficient for STAT reporter activation and atrophy in myotubes. In vivo, an increase in serum LIF preceded the increase in IL-6 in mice with C26 tumors. Overexpression of a dominant negative Stat3Cβ-EGFP gene in myotubes and in mouse muscle blocked the atrophy caused by C26 CM or C26 tumors, respectively. Taken together these data support an important role of LIF- JAK2-STAT3 in C26 cachexia and point to a therapeutic approach for at least some types of cancer cachexia. from three replicate wells of cells at each treatment, pools of total RNA were used to create cDNA which were evaluated on Affymetrix mouse gene 1.0 ST v.1 arrays.

2015-06-25 | E-GEOD-68827 | biostudies-arrayexpress

Genome-wide analysis of skeletal muscle gene expression in mice with C26 tumor-induced muscle wasting and cachexia

Project description:Analysis of gene expression in CD2F1 mice with cancer cachexia due to implantation of C26 colon carcinoma cells. Total RNA obtained from quadriceps muscle of female CD2F1 mice with early weight loss (10%) or severe weight loss (15%) due to intrascapular subcutaneous implantation of one million C26 colon adenocarcinoma cells.

2011-08-29 | E-GEOD-24112 | biostudies-arrayexpress

Leukemia Inhibitory Factor in C26 Cancer Cachexia

2015-06-25 | GSE68827 | GEO

Expression data of cardiac and gastrocnemius muscles of tumor bearing C26 mouse

Project description:We surveyed the transcriptomes of the whole heart and whole gastrocnemius muscle taken from two different types of Balb/c-DBAj hybrid mice (10-11 weeks old). The colon cancer bearing mice are called C26. The NTB are the non-tumor bearing mice. This dataset includes Affymetrix expression data collected from the biological triplicates of heart and gastrocnemius from both C26 and NTB mice.

2014-11-10 | GSE63032 | GEO

Exosomal miRNAs differences analysis between C26 and MC38

Project description:MiRNAs contained in C26-derived and MC38-derived exosomes were determined by IlluminaHiSeq platform. High-throughput sequencing was conducted at Shanghai MajorbioBiopharm TechnologyCo., Ltd. (Shanghai, China). In brief, total RNA from exosomes was prepared and quantified with a NanoDrop ND-2000(NanoDrop Technologies).RNA integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies,Santa Clara, CA, USA). A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Small RNA adapters were then ligated to the 5’ and 3’ ends of total RNA. After cDNA synthesis and amplification, the PCR-amplified fragments were purified from the PAGE gel, and the completed cDNA libraries were quantified by an Agilent 2100 Bioanalyzer. Cluster generation was performed on an IlluminacBot, and sequencing was performed on an IlluminaHiSeq 2000 platform and 50bp single-end reads were generated.The expression level of each miRNA was calculated according to the transcripts per million reads (TPM) method. Significant differently expressed (DE) miRNAs were extracted with |log2FC|>1 and FDR < 0.05 by DEseq2.

2021-04-24 | GSE173202 | GEO

Next Generation Sequencing of mRNA of cachectic mice muscles in C26 model

Project description:Cancer cachexia is a metabolic multifactorial syndrome that causes up to 20% of cancer-related deaths. Muscle atrophy, the hallmark of cancer cachexia, strongly impairs the quality of life of cancer patients; however, the underlying pathological process is still poorly understood. In our study, the transcriptome of cachectic gastrocnemius muscle in the C26 xenograft model was comparied with normal control. The key pathways involving this diseases was discovered according to the different expressed genes.

2021-05-27 | GSE175570 | GEO

Genome-wide analysis of skeletal muscle gene expression in mice with C26 tumor-induced muscle wasting and cachexia

Project description:Analysis of gene expression in CD2F1 mice with cancer cachexia due to implantation of C26 colon carcinoma cells.

2011-08-30 | GSE24112 | GEO

Myroides odoratimimus strain:C26-4_Circular

Project description:Myroides odoratimimus strain:C26-4_Circular Genome sequencing

| PRJNA1224307 | ENA