Genomics

Dataset Information

0

RNA immunoprecipitation (RIP)-Chip analysis for EWS-bound mRNA


ABSTRACT: Although EWS/FLI-1 fusion protein is responsible for most Ewing’s sarcoma family tumors (ESFT), the function of native EWS remains largely unknown. Here, we first showed that EWS repressed protein expression in a tethering assay. mRNAs bound to EWS were determined by RNA-immunoprecipitation Chip assay, and one of them, proline-rich Akt substrate of 40 kDa (PRAS40) mRNA, directly interacted with EWS. The inhibitor of AKT, API-2, repressed ESFT cell proliferation. We demonstrate that EWS negatively regulated PRAS40 protein expression through binding to PRAS40 3’UTR. Furthermore, PRAS40 knockdown inhibited the proliferation and metastatic potential of ESFT cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29313 | GEO | 2012/01/16

SECONDARY ACCESSION(S): PRJNA138649

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-29313 | biostudies-arrayexpress
| E-GEOD-31215 | biostudies-arrayexpress
2011-12-27 | GSE29085 | GEO
2011-08-05 | GSE31215 | GEO
2009-04-30 | GSE14543 | GEO
2017-11-09 | GSE99959 | GEO
2017-10-22 | GSE94502 | GEO
2017-10-22 | GSE94480 | GEO
2010-06-15 | GSE21511 | GEO
2020-05-12 | GSE144651 | GEO