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YKT6 promotes bladder cancer progression by stabilizing β-Catenin via USP7-mediated deubiquitination

ABSTRACT: Bladder cancer (BLCA) remains a highly lethal genitourinary malignancy with complex tumor biology and limited therapeutic strategies. This study investigates the oncogenic role of YKT6, a SNARE protein, in BLCA progression and molecular mechanisms. We demonstrate that YKT6 is significantly upregulated in BLCA tissues and cell lines, correlating with advanced tumor grade, aggressive histology, and poor patient prognosis. Comprehensive transcriptomic and functional analyses reveal that YKT6 promotes BLCA cell proliferation, migration, and metastasis both in vitro and in vivo. Mechanistically, YKT6 activates the Wnt/β-catenin signaling pathway through a novel mechanism involving USP7-mediated deubiquitination of β-catenin. By recruiting USP7, YKT6 inhibits β-catenin’s proteasomal degradation, thereby stabilizing the protein and driving nuclear accumulation. This stabilization leads to increased expression of oncogenic target genes and induces epithelial-mesenchymal transition (EMT). Pharmacological interventions demonstrated that Wnt signaling inhibition reverses YKT6-driven malignant effects, while pathway activation restores tumor progression in YKT6-silenced cells. USP7 knockdown abrogates YKT6-mediated β-catenin stabilization, confirming their functional interdependence. Clinically, the YKT6/USP7/β-catenin axis strongly correlates with poor prognosis, highlighting its potential as a diagnostic and therapeutic target. Our findings unveil YKT6 as a novel regulator of Wnt signaling through USP7-dependent deubiquitination, offering promising insights for precision BLCA therapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE293406 | GEO | 2025/11/27

REPOSITORIES: GEO

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Project description:Lee2003 - Roles of APC and Axin in Wnt Pathway (without regulatory loop) This model is described in the article: The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway. Lee E, Salic A, Krüger R, Heinrich R, Kirschner MW. PLoS Biol. 2003 Oct; 1(1): E10 Abstract: Wnt signaling plays an important role in both oncogenesis and development. Activation of the Wnt pathway results in stabilization of the transcriptional coactivator beta-catenin. Recent studies have demonstrated that axin, which coordinates beta-catenin degradation, is itself degraded. Although the key molecules required for transducing a Wnt signal have been identified, a quantitative understanding of this pathway has been lacking. We have developed a mathematical model for the canonical Wnt pathway that describes the interactions among the core components: Wnt, Frizzled, Dishevelled, GSK3beta, APC, axin, beta-catenin, and TCF. Using a system of differential equations, the model incorporates the kinetics of protein-protein interactions, protein synthesis/degradation, and phosphorylation/dephosphorylation. We initially defined a reference state of kinetic, thermodynamic, and flux data from experiments using Xenopus extracts. Predictions based on the analysis of the reference state were used iteratively to develop a more refined model from which we analyzed the effects of prolonged and transient Wnt stimulation on beta-catenin and axin turnover. We predict several unusual features of the Wnt pathway, some of which we tested experimentally. An insight from our model, which we confirmed experimentally, is that the two scaffold proteins axin and APC promote the formation of degradation complexes in very different ways. We can also explain the importance of axin degradation in amplifying and sharpening the Wnt signal, and we show that the dependence of axin degradation on APC is an essential part of an unappreciated regulatory loop that prevents the accumulation of beta-catenin at decreased APC concentrations. By applying control analysis to our mathematical model, we demonstrate the modular design, sensitivity, and robustness of the Wnt pathway and derive an explicit expression for tumor suppression and oncogenicity. This model is hosted on BioModels Database and identified by: BIOMD0000000658. To cite BioModels Database, please use: Chelliah V et al. BioModels: ten-year anniversary. Nucl. Acids Res. 2015, 43(Database issue):D542-8. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

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YKT6 promotes bladder cancer progression by stabilizing β-Catenin via USP7-mediated deubiquitination

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