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Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells

ABSTRACT: Analysis of BMP-2 induced gene expression of C2C12 cells treated with the endocytosis inhibitor dynasore to identify gene expression profiles which are sensitive to endocytosis inhibition. Our data highlight the importance of endocytosis for transmission of an extracellular BMP-2 signal into the nucleus to activate a transcriptional gene network

ORGANISM(S): Mus musculus

PROVIDER: GSE29373 | GEO | 2012/02/16

SECONDARY ACCESSION(S): PRJNA140215

REPOSITORIES: GEO

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Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells.

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Genome-wide analysis of endocytosis inhibition on BMP-2 induced gene expression in C2C12 cells

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2012-02-16 | E-GEOD-29373 | biostudies-arrayexpress

Quantitative proteomics of the SMAD transcription factor family reveals IPO5 as a BMP Receptor-SMAD specific importin

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Mouse C2C12 cells treated with/without hBMP2

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Ventromorphins: A new class of small molecule activators of the canonical BMP signaling pathway

Project description:Here we describe three new small-molecule activators of BMP signaling found by high throughput screening of a library of ~600,000 small molecules. Using a cell-based luciferase assay in the BMP4-responsive human cervical carcinoma clonal cell line, C33A-2D2, we identified three compounds with similar chemotypes, each ventralized zebrafish embryos and stimulated increased expression of the Bmp target genes, bmp2b and szl. Because these compounds ventralize zebrafish embryos, we have termed them “ventromorphins.” As expected for a BMP pathway activator, they induce the differentiation of C2C12 myoblasts to osteoblasts. Affymetrix RNA analysis confirmed the differentiation results and showed that ventromorphin treatments elicits a genetic response similar to BMP-4 treatment. Unlike isoliquiritigenin (SJ000286273), a flavone that maximally activates the pathway after 24 hours of treatment, all three ventromorphins induced SMAD1/5/8 phosphorylation within 30 minutes of treatment and achieved peak activity at 30 minutes or 1 hour, indicating that their direct responses are consistent with activated BMP signaling.

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LaminB1 DamID in undifferentiated mouse C2C12 myoblasts (ATCC, Lot 59501261) and differentiated C2C12 myotubes

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