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Gene profiling of viral sirna in mutants at generation g1.

ABSTRACT: gnp06-03_microtrac - gene profiling of viral sirna in mutants at generation g1. Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants?

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE29387 | GEO | 2011/05/25

SECONDARY ACCESSION(S): PRJNA140033

REPOSITORIES: GEO

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gene profiling of viral sirna in mutants at generation g1.

Project description:gnp06-03_microtrac - gene profiling of viral sirna in mutants at generation g1. Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? 4 dye-swap - gene knock out,treated vs untreated comparison

2011-05-24 | E-GEOD-29387 | biostudies-arrayexpress

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Project description:rs10-05_tcv - gene profiling of turnip crinkle virus (tcv) sirna - 1. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in A.thaliana? 2. There are also differentially regulated during an evolution and a fitness process? - This is a plant evolution project on TCV in which, the biological questions are: 1. What are the genes (including miRNA precursors) that are differentially regulated in Col0 and dcl234 mutant in wt conditions? 2. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in Col0 and dcl234 mutant? 3. There are also differentially regulated between the plant generations 1 (G1) and 11 (G11)? 4. What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA after fitness experiment? 24 dye-swap - gene knock out,treated vs untreated comparison

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Transcription profiling of Arabidopsis protoplasts transfected with TCV or HCRSV and their coat protein mutants

Project description:Common transcriptional responses of Arabidopsis thaliana protoplasts transfected with turnip crinkle virus (TCV) , hibiscus chlorotic ringspot virus (HCRSV) and their coat protein mutants.

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Project description:Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants at generation G1 and G11

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Project description:gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO n°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20°C.

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Comparative sRNA-Seq analysis of TCV-infected wildtype and dcl1-9 mutant Arabidopsis thaliana

Project description:Belonging to the Carmovirus family, Turnip crinkle virus (TCV) is a positive-strand RNA virus that can infect Arabidopsis. Most Arabidopsis ecotypes are highly susceptible to TCV, except for the TCV resistant line Di-17 derived from ecotype Dijon. Previous studies showed that many of the stress related genes have changed significantly after TCV infection. Besides the virus-triggered genes, small RNAs also play critical roles in plant defense by triggering either transcriptional and/or post-transcriptional gene silencing. In this study, TCV-infected wildtype Arabidopsis thaliana and dcl1-9 mutant plants were subjected to transcriptome and small RNA analysis to investigate the role of DCL1 in virus defense network.

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